11718096001
Roche
DAB Substrate
dark brown, /black precipitate; Visually evaluated
Szinonimák:
3,3′-diaminobenzidine tetrahydrochloride, DAB
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352204
Javasolt termékek
gyártó/kereskedő neve
Roche
Minőségi szint
szín
dark brown, /black precipitate; Visually evaluated
tárolási hőmérséklet
−20°C
Általános leírás
The metal enhanced DAB Substrate utilizes cobalt chloride and nickel chloride in a special formulation to produce a dark brown/black precipitate in the presence of horseradish peroxidase (HRP).
3, 3′ diaminobenzidine tetrahydrochloride (DAB) is a carcinogen that should be used safely. It is one of the major sensitive substrates for HRP (horseradish peroxidase). DAB is brown or amber in color and becomes insoluble and precipitates over the antigen-antibody site due to the presence of hydrogen peroxide and peroxidase.
3, 3′ diaminobenzidine tetrahydrochloride (DAB) is a carcinogen that should be used safely. It is one of the major sensitive substrates for HRP (horseradish peroxidase). DAB is brown or amber in color and becomes insoluble and precipitates over the antigen-antibody site due to the presence of hydrogen peroxide and peroxidase.
Alkalmazás
- DAB Substrate is a color sustrate for peroxidases and is used in a variety of applications:Immunohistocytochemistry
- In situ hybridization
- Western blot
- Dot blot
DAB substrate has been used in immunofluorescence and western blot.
Kiszerelés
1 set containing 2 components.
Elkészítési megjegyzés
Working solution:
Storage conditions (working solution): 2 to 8 °C
For best results, store the working solution at 2 to 8 °C when not using.
- Determine the volume of substrate required for the development of peroxidase.
- Remove the DAB/metal concentrate (10x) from -15 to -25 °C storage.
Note: Do not bring to 15 to 25 °C. - Dilute the DAB/metal concentrate (10x) with the peroxide buffer to a 1x working solution. For example, if 5 ml of substrate are required, dilute 500 μl of the DAB/metal concentrate (10x) with 4.5 ml of the Peroxide buffer.
- Mix well.
- Add the 1x working solution to the tissue or blot until desired substrate development.
Note: The 1x solution is stable over many hours.
For best results, store the working solution at 2 to 8 °C when not using.
Storage conditions (working solution): 2 to 8 °C
For best results, store the working solution at 2 to 8 °C when not using.
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Leírás
- DAB/metal concentrate, 25 ml 10x concentrated
- Peroxide Buffer, 250 ml
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 4 - Aquatic Chronic 2 - Carc. 1A - Carc. 1B - Flam. Liq. 2 Oral - Repr. 1B - Skin Sens. 1 Inhalation - STOT RE 2 - STOT SE 1
Tárolási osztály kódja
3 - Flammable liquids
WGK
WGK 3
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
Genetic deletion of Wdr13 improves the metabolic phenotype of Lepr db/db mice by modulating AP1 and PPARγ target genes.
Singh V P, et al.
Diabetologia, 58(2), 384-392 (2015)
Isabel Cristina Cañeda-Guzmán et al.
PloS one, 9(11), e112410-e112410 (2014-11-15)
Leishmania mexicana causes localized (LCL) or diffuse cutaneous leishmaniasis (DCL). The cause of dissemination in DCL remains unknown, yet NK cells possibly play a role in activating leishmanicidal mechanisms during innate and adaptive immune responses. We had previously shown that
Pathobiology of Human Disease: A Dynamic Encyclopedia of Disease Mechanisms (2014)
The developmental origin of zygodactyl feet and its possible loss in the evolution of Passeriformes.
Botelho J F, et al.
Proc. Royal Soc. Lond. B., 281(1788), 20140765-20140765 (2014)
The developmental origin of zygodactyl feet and its possible loss in the evolution of Passeriformes.
João Francisco Botelho et al.
Proceedings. Biological sciences, 281(1788), 20140765-20140765 (2014-06-27)
The zygodactyl orientation of toes (digits II and III pointing forwards, digits I and IV pointing backwards) evolved independently in different extant bird taxa. To understand the origin of this trait in modern birds, we investigated the development of the
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással