Összes fotó(1)
Fontos dokumentumok
11684302001
Roche
ABTS™ Solution
solution (ready-to-use), suitable for ELISA
Szinonimák:
ABTS™ Solution, ABTS
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352204
Javasolt termékek
Forma
solution (ready-to-use)
Minőségi szint
használat
sufficient for 1,500-3,000 assays (ELISA)
kiszerelés
pkg of 3 × 100 mL
gyártó/kereskedő neve
Roche
technika/technikák
ELISA: suitable
tárolási hőmérséklet
2-8°C
Általános leírás
ABTS™ Solution is a ready to use substrate for peroxidase-driven indicator reactions. It contains 2,2-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and H2O2 in glycin/citric acid buffer and is available in slightly green color.
Alkalmazás
ABTS Solution is a chromogenic substrate for peroxidase in ELISA assays.
Biokémiai/fiziológiai hatások
2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS™ acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS™ is a highly sensitive, specific and reproducible technique This solution is an excellent substrate for enzyme immunoassays with horseradish peroxidase as a marker enzyme.
Fizikai forma
Reaction product:
Color: green
Evaluation: photometric (405nm)
Color: green
Evaluation: photometric (405nm)
Analízis megjegyzés
Absorption: ABTS absorption spectrum reference must always be measured and is determined by a spectrum. The Reference wavelength of 490 nm or higher should be selected due to no measureable absorption. The Reference absorption is usually automatically subtracted by the Plate Reader (background).
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Jogi információk
ABTS is a trademark of Roche
WGK
nwg
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
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