Összes fotó(1)
Fontos dokumentumok
11207733910
Roche
Anti-Digoxigenin-POD, Fab fragments
from sheep
Szinonimák:
anti-digoxigenin, digoxigenin
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
klón:
polyclonal
application:
citations:
339
Javasolt termékek
biológiai forrás
sheep
Minőségi szint
konjugátum
peroxidase conjugate
antitest forma
F(ab′)2 fragment of affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
Forma
lyophilized (clear, brown solution after reconstitution)
kiszerelés
pkg of 150 U
gyártó/kereskedő neve
Roche
tárolási hőmérséklet
2-8°C
Általános leírás
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from an anti-digoxigenin antibody, conjugated with horse-radish peroxidase (POD). Anti-Digoxigenin-POD, Fab fragments is useful for the detection of digoxigenin-labeled compounds.
Egyediség
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Alkalmazás
Use Anti-Digoxigenin-POD, Fab fragments for the detection of digoxigenin-labeled compounds using:
- Dot blot
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
Elkészítési megjegyzés
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: Dot blot: 150 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: Dot blot: 150 mU/ml
- ELISA: 50 to 150 mU/ml
- Immunohistocytochemistry: 250 to 500 mU/ml
- In situ hybridization: 1.5 to 7.5 U/ml
- Southern blot: 150 mU/ml
- Western blot: 250 to 500 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Feloldás
Add 1 ml double-distilled water to a final concentration of 150 U/ml.
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
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Figyelmeztetés
Warning
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Skin Sens. 1
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 1
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
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Cikkek
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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