Összes fotó(1)
Fontos dokumentumok
11685619910
Roche
Fluorescein RNA Labeling Mix
solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization
Szinonimák:
RNA labeling
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41105500
Javasolt termékek
form
solution
Minőségi szint
használat
sufficient for 20 reactions
kiszerelés
pkg of 40 μL
gyártó/kereskedő neve
Roche
technika/technikák
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
tárolási hőmérséklet
−20°C
Általános leírás
Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).
Egyediség
Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).
Alkalmazás
Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
- in situ hybridization to tissues
- Southern blots
- northern blots
- plaque or colony lifts
- RNase protection experiments
Minőség
The mix is function tested in combination with the DIG RNA Labeling Kit.
Elv
Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.
Elkészítési megjegyzés
10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
nwg
Lobbanási pont (F)
No data available
Lobbanási pont (C)
No data available
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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