Összes fotó(2)
Fontos dokumentumok
11093070910
Roche
Biotin-16-dUTP
Szinonimák:
Bio-16-dUTP tetralithium salt, Biotin-labeled Uridines, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′-deoxyuridine 5′-triphosphate
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
Tapasztalati képlet (Hill-képlet):
C32H52N7O18P3S
CAS-szám:
Molekulatömeg:
947.78
UNSPSC kód:
41116100
Javasolt termékek
form
solution
Minőségi szint
kiszerelés
pkg of 50 μL (50 nmol; 1mM)
gyártó/kereskedő neve
Roche
szín
colorless
oldhatóság
water: miscible
tárolási hőmérséklet
−20°C
Looking for similar products? Látogasson el ide Útmutató a termékösszehasonlításhoz
Általános leírás
Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.Biotin-16-dUTP is used to produce biotinylated DNA probes that are commonly used to detect specific DNA and RNA sequences in different methods like Southern and Northern blotting, in situ hybridization, and sandwich assays.
Alkalmazás
Biotin-16-dUTP has been used:
- For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation. Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
- as a substrate for terminal transferase, DNA polymerase I (holoenzyme and Klenow fragment), Taq DNA polymerase, and reverse transcriptase (e.g., Transcriptor). The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling
- In fluorescence in situ hybridization (FISH)
Biokémiai/fiziológiai hatások
Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.
Minőség
Typical analysis: At least 85% deoxynucleoside triphosphate (Biotin-16-dUTP) (HPLC, area%).
Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial).
Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial).
Analízis megjegyzés
Absorption: The spectral data of Biotin-16-dUTP (at pH 7.3):
Emax241: E = 10.700;
Emax292: E = 7.000;
Emax265: E = 4.400;
A292/265 : 1.6.
Emax241: E = 10.700;
Emax292: E = 7.000;
Emax265: E = 4.400;
A292/265 : 1.6.
Egyéb megjegyzések
Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate- Biotin Luminescence Detection Kit.
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
nwg
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
Sudhanshu Agrawal et al.
American journal of physiology. Cell physiology, 311(3), C386-C391 (2016-07-15)
The water-soluble biotin (vitamin B7) is indispensable for normal human health. The vitamin acts as a cofactor for five carboxylases that are critical for fatty acid, glucose, and amino acid metabolism. Biotin deficiency is associated with various diseases, and mice
Ling Hu et al.
Frontiers in cell and developmental biology, 9, 645368-645368 (2021-07-09)
Schizophrenia (SCZ) is a chronic and severe mental disease that affects around 1% of the population. The precise etiology of SCZ still remains largely unknown, and no conclusive mechanisms are firmly established. Recent advances in epidemiological and clinical investigation support
Arnaud Augert et al.
Free radical biology & medicine, 65, 969-977 (2013-09-03)
Little is known about the biological functions of the phospholipase A2 receptor (PLA2R1) except that it has the ability to bind a few secreted phospholipases A2 (sPLA2's). We have previously shown that PLA2R1 regulates senescence in normal human cells. In
Synthesis in vitro and application of biotinylated DNA probes for human papilloma virus type 16 by utilizing the polymerase chain reaction
DAY PJR
Biochemical Methods, 267, 119-123 (1990)
Di Hu et al.
Journal of neurotrauma, 37(21), 2244-2260 (2020-06-20)
Individuals with spinal cord injury (SCI) often develop debilitating neuropathic pain, which may be driven by neuronal damage and neuroinflammation. We have previously demonstrated that treatment using 670 nm (red) light irradiation alters microglia/macrophage responses and alleviates mechanical hypersensitivity at 7
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással