11088939001

Tumor Necrosis Factor-α, human (hTNF-α)

recombinant (yeast)

• Szinonimák: hTNF, human necrosis factor
• UNSPSC kód: 12352207

Tulajdonságok

• biológiai forrás: human
• Minőségi szint: 100
• rekombináns: expressed in yeast
• Teszt: ≥95% (SDS-PAGE)
• Forma: solution
• hatékonyság: ≤0.01 ng/mL EC₅₀
• specifikus aktivitás: >1*10^8 U/mg
• molekulatömeg: 17,000 Da
• kiszerelés: pkg of 1,000,000 U (10 μg, 1ml)
• gyártó/kereskedő neve: Roche
• tárolási körülmény: avoid repeated freeze/thaw cycles
• szennyeződések: <0.1 EU/μgtested (LAL test)
• UniProt elérési szám: Q9UBM5
• tárolási hőmérséklet: −20°C
• Géninformáció: human ... TNF(7124)

Leírás

Általános leírás

>1 x 10^⊥8 U/mg, <0.01 ng/ml (hTNF-a, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.

Egyediség

Human hTNF-α is effective on human and mouse cells.

Species specificity: human, mouse

Alkalmazás

Tumor Necrosis Factor-α (hTNF-α)

• Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.
• Has activating and growth stimulating activities on a variety of normal cells.
• Has antiviral activity on many cell types in vitro.
• Can cause tumor necrosis of certain tumors in vivo.

Biokémiai/fiziológiai hatások

Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.

Minőség

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

Szekvencia

Chain Length 157 AA

One polypeptide chain (157 amino acids), identical to natural hTNF-α.

Egység definíció

EC₅₀ definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).

Fizikai forma

Solution, filtered through 0.2 μm pore size membrane.

Elkészítési megjegyzés

Working concentration: 1 ng/ml
For complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.
Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: nwg
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash