10197734001

Glutamate Dehydrogenase (GlDH)

from beef liver

• Szinonimák: DNA marker
• Enzyme Commission szám: 1.4.1.3 (BRENDA, IUBMB)
• UNSPSC kód: 12352204

Tulajdonságok

• biológiai forrás: bovine liver
• Minőségi szint: 100
• form: lyophilized
• specifikus aktivitás: 10 U/mg; ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
• kiszerelés: pkg of 3,000 U
• gyártó/kereskedő neve: Roche
• koncentráció: ≥10-20 % (w/w)
• technika/technikák: activity assay: suitable
• szín: white
• optimális pH: 8
• oldhatóság: water: 20 mg/mL, colorless
• alkalmasság: suitable for UV spectrophotometry and general use
• UniProt elérési szám: P00366
• alkalmazás(ok): life science and biopharma
• idegen aktivitás: ADH <0.00500%; LDH <0.00500%; MDH <0.00500%
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C
• Géninformáció: bovine ... GLUD1(281785)

Leírás

Általános leírás

Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.

GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria. It acts as a branch-point enzyme between amino acid oxidation and urea production. L-glutamate:NAD(P)⁺ oxidoreductase is involved in deamination.

Alkalmazás

Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.

Minőség

Contaminants: <0.005% ADH, LDH, and MDH, each

Szekvencia

The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD).

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash