ST1200
Anti-Ubiquitin Mouse mAb (FK2)
liquid, clone FK2, Calbiochem®
Szinonimák:
Mouse Anti-Ubiquitin, Ubiquitin Detection Antibody
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
NACRES:
NA.41
klón:
FK2, monoclonal
application:
citations:
53
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
unpurified
antitest terméktípus
primary antibodies
klón
FK2, monoclonal
Forma
liquid
tartalmaz
≤0.1% sodium azide as preservative
faj reaktivitás (homológia által előrejelzett)
all
gyártó/kereskedő neve
Calbiochem®
tárolási körülmény
OK to freeze
avoid repeated freeze/thaw cycles
izotípus
IgG1
kiszállítva
wet ice
tárolási hőmérséklet
−20°C
célzott transzláció utáni módosítás
unmodified
Általános leírás
Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins.
Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.
Immunogén
human
unpurified polyubiquitinylated lysozyme
Alkalmazás
ELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)
Kiszerelés
Please refer to vial label for lot-specific concentration.
Figyelmeztetés
Toxicity: Standard Handling (A)
Fizikai forma
In PBS.
Elkészítési megjegyzés
Raji cells
Feloldás
Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
Egyéb megjegyzések
Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
Matsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Jogi információk
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Amijai Saragovi et al.
eLife, 9 (2020-11-24)
Systemic oxygen restriction (SOR) is prevalent in numerous clinical conditions, including chronic obstructive pulmonary disease (COPD), and is associated with increased susceptibility to viral infections. However, the influence of SOR on T cell immunity remains uncharacterized. Here we show the
Yin Wu et al.
The Journal of biological chemistry, 291(23), 12370-12382 (2016-04-30)
Sepsis is one of the leading causes of death worldwide. Although the prevailing theory for the sepsis syndrome is a condition of uncontrolled inflammation in response to infection, sepsis is increasingly being recognized as an immunosuppressive state known as endotoxin
Tapas Mukherjee et al.
The Journal of biological chemistry, 296, 100050-100050 (2020-11-11)
Large cytosolic protein aggregates are removed by two main cellular processes, autophagy and the ubiquitin-proteasome system, and defective clearance of these protein aggregates results in proteotoxicity and cell death. Recently, we found that the eIF2α kinase heme-regulated inhibitory (HRI) induced
Yan-Ning Rui et al.
Autophagy, 11(5), 812-832 (2015-05-20)
By monitoring the fragmentation of a GST-BHMT (a protein fusion of glutathionine S-transferase N-terminal to betaine-homocysteine S-methyltransferase) reporter in lysosomes, the GST-BHMT assay has previously been established as an endpoint, cargo-based assay for starvation-induced autophagy that is largely nonselective. Here
Juan Wang et al.
The Plant cell, 32(2), 414-428 (2019-12-13)
UBL-UBA (ubiquitin-like-ubiquitin-associated) proteins are ubiquitin receptors and transporters in the ubiquitin-proteasome system that play key roles in plant growth and development. High salinity restricts plant growth by disrupting cellular metabolism, but whether UBL-UBA proteins are involved in this process is
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