NA61
Anti-BrdU (Ab-3) Mouse mAb (Mobu-1)
liquid, clone Mobu-1, Calbiochem®
Szinonimák:
Anti-Bromodeoxyuridine
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
NACRES:
NA.43
klón:
Mobu-1, monoclonal
application:
citations:
35
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
Mobu-1, monoclonal
Forma
liquid
tartalmaz
≤0.1% sodium azide as preservative
faj reaktivitás (homológia által előrejelzett)
all
gyártó/kereskedő neve
Calbiochem®
tárolási körülmény
do not freeze
izotípus
IgG1
kiszállítva
wet ice
tárolási hőmérséklet
2-8°C
célzott transzláció utáni módosítás
unmodified
Általános leírás
Anti-BrdU (Ab-3), mouse monoclonal, clone Mobu-1, recognizes BrdU. Requires biosynthetic labeling of the target cells with BrdU. It is validated for ICC and use with frozen and paraffin sections.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3-X63-Ag8-653 mouse myeloma cells (see application references). Recognizes BrdU (bromodeoxyuridine).
Recognizes BrdU (bromodeoxyuridine). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine. This is the preferred BrdU antibody for staining.
Immunogén
BrdU-labeled DNA
Alkalmazás
Frozen Sections (2 g/ml)
Immunocytochemistry (1 g/ml)
Paraffin Sections (2.5 g/ml, trysin pre-treatment required)
Immunoblotting (not recommended)
Kiszerelés
Please refer to vial label for lot-specific concentration.
Figyelmeztetés
Toxicity: Standard Handling (A)
Fizikai forma
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Analízis megjegyzés
Negative Control
Unlabeled cells
Unlabeled cells
Positive Control
BrdU labeled DNA
BrdU labeled DNA
Egyéb megjegyzések
Gratzner, H.G. 1982. Science218, 474.
Gratzner, H.G. and Leif, R.C. 1981. Cytometry1, 385.
Gratzner, H.G. et al. 1975. Exp. Cell Res.95, 88.
Gratzner, H.G. and Leif, R.C. 1981. Cytometry1, 385.
Gratzner, H.G. et al. 1975. Exp. Cell Res.95, 88.
To enable antibody binding to the incorporated BrdU, cells must be fixed, permeabilized, and the DNA denatured. This is done in one step by treatment with Fixative/Denaturing Solution (70% EtOH, 0.1 N NaOH). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine (Cat. No. 203806). Cultured cells can be pulse labeled with 10 µM bromodeoxyuridine for 30 minutes. For whole tissues, animals can be injected with 50 mg BrdU/kg body weight, sacrificed 1 h later, and the target organ(s) removed. Antibody should be titrated for optimal results in individual system.
Jogi információk
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Cancer research, 66(9), 4715-4724 (2006-05-03)
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Transgenic expression of the human growth hormone minigene promotes pancreatic β-cell proliferation.
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American journal of physiology. Regulatory, integrative and comparative physiology, 309(7), R788-R794 (2015-07-24)
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