MAB3299
Anti-DNA Antibody, single stranded specific, clone F7-26
clone F7-26, Chemicon®, from mouse
Szinonimák:
Anti-single stranded DNA, Single-stranded DNA antibody
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(12)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
F7-26, monoclonal
application:
FACS
ICC
IHC (p)
ICC
IHC (p)
technika/technikák:
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
citations:
35
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
F7-26, monoclonal
faj reaktivitás (homológia által előrejelzett)
all
gyártó/kereskedő neve
Chemicon®
technika/technikák
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
izotípus
IgM
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Általános leírás
Apoptosis is a genetically and biochemically regulated mechanism of programmed cell death that plays an important role in a variety of human diseases including cancer, immune disorders, and neurologic, cardiovascular and infectious diseases. Decreased stability of apoptotic DNA toward thermal denaturation is induced by the proteolysis of DNA-bound proteins during the execution of apoptosis. This conclusion is supported by the binding of anti-ssDNA monoclonal antibodies to non-apoptotic cells treated with proteinase K before heating and by prevention of DNA denaturation and MAb reactivity in apoptotic nuclei reconstituted with histones (Frankfurt et al. 1996, 1997). Thus, staining of cell suspensions and tissue sections with such antibodies following a heat treatment, which induces DNA denaturation in situ only in apoptotic nuclei, is a specific and sensitive method for the detection of apoptotic cells. CHEMICON MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types.
Egyediség
Specifically reactive with single-stranded DNA and is ideal for the detection of apoptotic cells in suspension or in tissue sections. The antibody does not recognize DNA in double-stranded conformations. MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types. Antibody reacts specifically with deoxycytidine and requires stretch of ssDNA of at least 25-30 bases in length for the binding. Importantly, in contrast with the TUNEL method, monoclonal antibodies to ssDNA are specific for apoptotic cell death and do not detect necrotic cells.
Immunogén
Epitope: single stranded specific
F7-26 was generated by the immunization of mice with calf thymus single-stranded DNA.
Alkalmazás
1. Immunoprecipitation
2. Immunohistochemistry: formalin fixed, paraffin embedded tissues & 4% PF frozen tissues (special detection protocol required. FACS analysis (special protocol required),
2. Immunohistochemistry: formalin fixed, paraffin embedded tissues & 4% PF frozen tissues (special detection protocol required. FACS analysis (special protocol required),
Anti-DNA Antibody, single stranded specific, clone F7-26 detects level of DNA & has been published & validated for use in FC, IC, IH, IH(P).
Fizikai forma
Format: Purified
Purified Immunoglobulin. Liquid in PBS supplemented with 5% fetal bovine Serum (FBS), containing no preservatives.
Tárolás és stabilitás
Maintain frozen at -80°C in undiluted aliquots for up to 12 months after date of purchase.
Analízis megjegyzés
Control
Apoptotic cells
Apoptotic cells
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Fumiko Kobayashi et al.
Infection and immunity, 79(12), 4791-4801 (2011-09-29)
Helicobacter pylori infection is associated with several autoimmune diseases, in which autoantibody-producing B cells must be activated. Among these B cells, CD5-positive B-1a cells from BALB/c mice were confirmed to secrete autoantibodies when cocultured with purified H. pylori urease in
Alan L Chang et al.
Free radical biology & medicine, 78, 179-189 (2014-12-03)
Oxidative mitochondrial damage is closely linked to inflammation and cell death, but low levels of reactive oxygen and nitrogen species serve as signals that involve mitochondrial repair and resolution of inflammation. More specifically, cytoprotection relies on the elimination of damaged
Simon J Fung et al.
Brain research, 1179, 42-50 (2007-09-25)
Patients with obstructive sleep apnea (OSA) exhibit hippocampal damage and cognitive deficits. To determine the effect of apnea on the synaptic transmission in the hippocampus, we performed electrophysiological studies in an in vivo guinea pig model of OSA. Specifically, we
Jia Chen et al.
Brain research. Molecular brain research, 134(2), 215-225 (2005-04-20)
The neuroprotective effects of Delta(9)-tetrahydrocannabinol (THC) were examined using an in vitro model in which the AF5 CNS cell line was exposed to toxic levels of N-methyl-d-aspartate (NMDA), an agonist of the NMDA glutamate receptor. NMDA toxicity was reduced by
Kiran Assi et al.
Immunology, 118(1), 112-121 (2006-04-25)
Stress-activated protein kinases (SAPKs) are activated in human inflammatory bowel disease (IBD). Recently it has been demonstrated that p38MAPK (mitogen-activated protein kinase) inhibition using SB203580 is effective in reducing disease in both dextran sulphate sodium (DSS)-induced and 2,4,6-trinitrobenzenesulphonic acid (TNBS)-induced
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással