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Merck
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MAB030

Sigma-Aldrich

Anti-DNA Antibody, double stranded, clone BV16-13

culture supernatant, clone BV16-13, Chemicon®

Szinonimák:

Double-stranded DNA antibody

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
BV16-13, monoclonal
application:
ELISA
ICC
RIA
technika/technikák:
ELISA: suitable
immunocytochemistry: suitable
radioimmunoassay: suitable
citations:
23

biológiai forrás

mouse

Minőségi szint

100

antitest forma

culture supernatant

antitest terméktípus

primary antibodies

klón

BV16-13, monoclonal

faj reaktivitás (homológia által előrejelzett)

all

gyártó/kereskedő neve

Chemicon®

technika/technikák

ELISA: suitable
immunocytochemistry: suitable
radioimmunoassay: suitable

izotípus

IgG2aκ

kiszállítva

wet ice

célzott transzláció utáni módosítás

unmodified

Egyediség

Double stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA.

BINDING ASSAY:

Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.



ANTIGEN BINDING

PROFILE: Relative Binding To:



Natural DNA Synthetic homopolymers

dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01



Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).



a) Native calf thymus DNA b) Heat-denatured calf thymus DNA

Immunogén

Epitope: double stranded, 28% reactive with single stranded

Alkalmazás

Detect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC.
EIA/RIA

Immunocytochemistry

Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair

Apoptosis - Additional

Fizikai forma

Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.

Tárolás és stabilitás

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Jogi információk

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 1

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Feng Huang et al.
Molecular medicine reports, 16(4), 4817-4822 (2017-08-10)
Lupus nephritis (LN) is a common complication of systemic lupus erythematosus. The present study aimed to elucidate the protective effect of magnolol (MG) on the progression of LN, via inhibition of key signaling pathways. The results of the present study
C A Cooke et al.
The Journal of cell biology, 120(5), 1083-1091 (1993-03-01)
The location of the cis-acting DNA sequences that direct the assembly of the mammalian kinetochore is not known. A variety of circumstantial evidence, however, has led to the widespread belief that they are present throughout the kinetochore including the kinetochore
Marc Lamphier et al.
Molecular pharmacology, 85(3), 429-440 (2013-12-18)
The discovery that circulating nucleic acid-containing complexes in the serum of autoimmune lupus patients can stimulate B cells and plasmacytoid dendritic cells via Toll-like receptors 7 and 9 suggested that agents that block these receptors might be useful therapeutics. We
Taeko Sasaki et al.
Scientific reports, 7(1), 11257-11257 (2017-09-14)
Mitochondrial DNA (mtDNA) is organized in nucleoprotein complexes called mitochondrial nucleoids (mt-nucleoids), which are critical units of mtDNA replication and transmission. In humans, several hundreds of mt-nucleoids exist in a cell. However, how numerous mt-nucleoids are maintained during the cell
B Zhang et al.
Nature, 390(6659), 477-484 (1997-12-11)
The nematode Caenorhabditis elegans has two sexes, males and hermaphrodites. Hermaphrodites Initially produce sperm but switch to producing oocytes. This switch appears to be controlled by the 3' untranslated region of fem-3 messenger RNA. We have now identified a binding
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