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AP1064

Sigma-Aldrich

PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb

liquid, Calbiochem®

Szinonimák:

Anti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352203
NACRES:
NA.41

biológiai forrás

rabbit

Minőségi szint

antitest forma

affinity isolated antibody

antitest terméktípus

primary antibodies

klón

polyclonal

form

liquid

tartalmaz

≤0.1% sodium azide as preservative

faj reaktivitás

human

gyártó/kereskedő neve

Calbiochem®

tárolási körülmény

OK to freeze
avoid repeated freeze/thaw cycles

kiszállítva

wet ice

tárolási hőmérséklet

−20°C

célzott transzláció utáni módosítás

phosphorylation (pSer300)

Általános leírás

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300.
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300 in HEK293 cells.
This PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).

Immunogen

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α

Alkalmazás



Immunoblotting (0.5 μg/ml)
Immunocytochemistry (1 μg/ml)

Kiszerelés

Please refer to vial label for lot-specific concentration.

Figyelmeztetés

Toxicity: Standard Handling (A)

Fizikai forma

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.

Feloldás

Following initial thaw, aliquot and freeze (-20°C).

Analízis megjegyzés

Negative Control
HEK293 cells treated with dichloroacetate
Positive Control
HEK293 cells

Egyéb megjegyzések

Due to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.
Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

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