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Merck
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Fontos dokumentumok

ABE135

Sigma-Aldrich

Anti-Sp1 Antibody

from rabbit

Szinonimák:

Sp1 transcription factor, specificity protein 1

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biológiai forrás

rabbit

Minőségi szint

antitest forma

purified antibody

antitest terméktípus

primary antibodies

klón

polyclonal

faj reaktivitás

mouse, human, rat

technika/technikák

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI elérési szám

UniProt elérési szám

kiszállítva

wet ice

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... SP1(6667)

Általános leírás

Sp1 was one of the first transcription factors to be characterized in mammalian cells. The Sp1 protein plays a role in the regulation of gene expression. This protein has three Cys2His2 zinc-finger motifs that have been shown to bind to the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ also known as a GC box. Sp1 is a ubiquitous transcription factor required for the expression of a variety of genes, such as those involved in the cell cycle and development. Interaction of the Sp1 protein with the GC box activates transcription of specific mRNAs from genes that contain functional recognition sites.

Egyediség

Homology to chimpanzee, canine, and rhesus monkey is 99%.
This antibody recognizes Sp1.

Immunogén

GST-tagged recombinant protein corresponding to full-length human Sp1.

Alkalmazás

Anti-Sp1 Antibody is a Rabbit Polyclonal Antibody for detection of Sp1 also known as Sp1 transcription factor, specificity protein 1 & has been validated in WB, IP, ICC.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Sp1 in HeLa and A431 cells.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Minőség

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Sp1 on 10 µg of HeLa nuclear extract.

Cél megnevezése

95 kDa and 105 kDa

Kapcsolódás

Replaces: MABE550

Fizikai forma

Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Analízis megjegyzés

Control
HeLa nuclear extract

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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