688002-M

Y-27632

≥95% (HPLC), liquid, Rho kinase inhibitor, Calbiochem^®

• Szinonimák: InSolution Y-27632 in DMSO, Rho Kinase Inhibitor VI, (R)-(+)- trans-N-(4-Pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide, 2HCl
• Tapasztalati képlet (Hill-képlet): C₁₄H₂₁N₃O · 2HCl · xH₂O
• Molekulatömeg: 320.26 (anhydrous basis)
• UNSPSC kód: 12352200

Tulajdonságok

• product name: Y-27632 in DMSO, InSolution, ≥95%, 10 mM, reversible and selective inhibitor of Rho-associated protein kinases
• Minőségi szint: 100
• Teszt: ≥95% (HPLC)
• form: liquid
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• tárolási hőmérséklet: −70°C

Leírás

Általános leírás

A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (K_i = 140 nM for p160^ROCK). Also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP. Exhibits 10- to 50-fold lower affinity for PKC_ε than p160^ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at higher concentrations (~100 µM). Also acts as a potent inhibitor of agonist-induced Ca²⁺ sensitization of myosin phosphorylation and smooth muscle contraction. Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency.

A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (K_i = 140 nM for p160^ROCK). Also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP. Exhibits 10- to 50-fold lower affinity for PKC_ε than p160^ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at higher concentrations (~100 µM). Also acts as a potent inhibitor of agonist-induced Ca²⁺ sensitization of myosin phosphorylation and smooth muscle contraction. Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency. A 5 mM (500 µg/296 µl) solution of Y-27632 (Cat. No. 688001) in H₂O and the solid form of this compound (Cat. No. 688000) are also available.

Figyelmeztetés

Toxicity: Irritant (B)

Fizikai forma

A 10 mM (1 mg/296 µl) solution of Y-27632 (Cat. No. 688000)in DMSO.

Feloldás

Following initial thaw, aliquot and freeze (-20°C).

Egyéb megjegyzések

Watanabe, K., et al. 2007. Nature Biotech.25, 681.
Chitaley, K., et al. 2001. Nat. Med.7, 119.
Davies, S.P., et al. 2000. Biochem. J. 351, 95.
Narumiya, S., et al. 2000. Methods Enzymol.325, 273.
Hirose, M., et al. 1998. J. Cell Biol.141, 1625.
Maekawa, M., et al. 1999. Science285, 895.
Uehata, M., et al. 1997. Nature389, 990.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 2
• Lobbanási pont (F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Lobbanási pont (C): 87 °C - closed cup - (Dimethylsulfoxide)