2384

Creatine Phosphokinase, Rabbit Skeletal Muscle

• Szinonimák: Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK
• CAS-szám: 9001-15-4
• Enzyme Commission szám: 2.7.3.2 (BRENDA, IUBMB)
• MDL-szám: MFCD00130876
• UNSPSC kód: 12352202
• NACRES: NA.21

Tulajdonságok

• biológiai forrás: rabbit skeletal muscle
• Minőségi szint: 200
• form: lyophilized
• specifikus aktivitás: ≥300 U/mg, 25 °C; 750 U/mg, 37 °C
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; desiccated (hygroscopic)
• oldhatóság: 0.1 M imidazole buffer, pH 6.6: soluble; 0.5% NaHCO₃, pH 9.0: soluble
• idegen aktivitás: ATPase, hexokinase, myokinase <0.001%
• kiszállítva: ambient
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

Native creatine phosphokinase from rabbit muscle. Catalyzes the conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Figyelmeztetés

Toxicity: Standard Handling (A)

Egység definíció

One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.

Feloldás

Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.

Egyéb megjegyzések

Bessman, S.P. 1987. Anal. Biochem.161, 519.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable