197217

Bcl-x_L BH4₄₋₂₃, Human, Cell-Permeable

A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity.

• Szinonimák: Bcl-x_L BH4₄₋₂₃, Human, Cell-Permeable, HIV-TAT₄₈₋₅₇-β-Ala-Bcl-x _L BH4₄₋₂₃, Ac-GRKKRRQRRR-βA-SNRELVVDFLSYKLSQKGYS-OH
• Tapasztalati képlet (Hill-képlet): C₁₆₅H₂₇₉N₅₉O₄₆
• Molekulatömeg: 3825.35
• UNSPSC kód: 12352202
• NACRES: NA.77

Tulajdonságok

• Minőségi szint: 100
• Teszt: ≥95% (HPLC)
• form: lyophilized
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; desiccated (hygroscopic)
• oldhatóság: 20% acetic acid: 5 mg/mL; DMSO: 5 mg/mL
• kiszállítva: ambient
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Leads to the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨ_m). Contains the conserved N-terminal homology domain (BH4) of Bcl-x_L (amino acids 4 - 23) that has been shown to be essential for inhibiting VDAC activity in liposomes and in isolated mitochondria. The BH4 domain is linked to a carrier peptide, a 10-amino acid HIV-TAT_48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized to the mitochondria.

A highly cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Ultimately results in the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨm). Contains the conserved N-terminal homology domain (BH4) of Bcl-x_L (amino acids 4-23) that has been shown to be essential for inhibiting VDAC activity in liposomes and isolated mitochondria. The BH4 domain is linked to the carrier peptide, the 10-amino acid HIV-TAT_48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized in the mitochondria.

Biokémiai/fiziológiai hatások

Cell permeable: yes

Primary Target
Voltage-dependent anion channel (VDAC)

Product does not compete with ATP.

Reversible: yes

Kiszerelés

Packaged under inert gas

Figyelmeztetés

Toxicity: Standard Handling (A)

Szekvencia

Ac-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-βAla-Ser-Asn-Arg-Glu-Leu-Val-Val-Asp-Phe-Leu-Ser-Tyr-Lys-Ser-Gln-Lys-Gly-Tyr-Ser-OH

Fizikai forma

Supplied as a trifluoroacetate salt.

Feloldás

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Egyéb megjegyzések

Chen m., et al. 2002. J. Biol. Chem. 277, 29181.
Futaki, S., et al. 2001. J. Biol. Chem.276, 5836.
Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA97, 3100.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable