The presence of FBS in the cell culture supernatant containing IgG2b may lead to reduced yield and purity of the mAb, IgG2b, as FBS contains bovine immunoglobulins that can compete with the target antibody for binding to the protein G column.
16-266
Protein G Agarose, Fast Flow
Protein G Agarose, Fast Flow suitable for medium and low pressure chromatography of IgG from mouse, sheep, and rabbit, and for immunoprecipitations.
Szinonimák:
Protein G resin
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Méret kiválasztása
10 ML
266 000,00 Ft
266 000,00 Ft
Szállítás becsült dátuma2025. február 03.részletek
Összes fotó(1)
Méret kiválasztása
Nézet módosítása
10 ML
266 000,00 Ft
About This Item
UNSPSC kód:
41116133
eCl@ss:
32160801
NACRES:
NA.56
266 000,00 Ft
Szállítás becsült dátuma2025. február 03.részletek
Javasolt termékek
Forma
liquid
gyártó/kereskedő neve
Upstate®
technika/technikák
affinity chromatography: suitable
immunoprecipitation (IP): suitable
western blot: suitable
kiszállítva
wet ice
Általános leírás
Fast flow agarose comprises highly crosslinked agarose beads or gels that can tolerate high pressures compared to standard agarose bead resins. Thus, these resins are ideal for fast protein liquid chromatography (FPLC) purification methods. They are preferred for medium to large-scale antibody purification. This product contains protein G covalently coupled by cyanogen bromide to highly cross-linked 4% agarose beads. It is stable in all aqueous buffers used in protein G chromatography.
Alkalmazás
Minőség
routinely evaluated by immunoprecipitation and purification
Fizikai forma
water containing 20% ethanol
Tárolás és stabilitás
1 year at 4°C
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Figyelmeztetés
Warning
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Flam. Liq. 3
Tárolási osztály kódja
3 - Flammable liquids
WGK
WGK 1
Lobbanási pont (F)
116.6 °F
Lobbanási pont (C)
47 °C
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Is the 16-266 protein G column compatible with purifying IgG in the presence of FBS when purifying mAb, IgG2b, from cell culture supernatant containing FBS?
1 answer-
Helpful?
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What is the approximate diameter of each agarose bead?
1 answer-
The diameter range of 4% beaded agarose typically falls between 50 to 165 micrometers.
Helpful?
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