05-235
Anti-EF1α Antibody, clone CBP-KK1
clone CBP-KK1, Upstate®, from mouse
Szinonimák:
CTCL tumor antigen, EF1a-like protein, Elongation factor 1 A-1, Elongation factor Tu, Leukocyte receptor cluster member 7, cervical cancer suppressor 3, elongation factor 1 alpha subunit, elongation factor 1-alpha, eukaryotic translation elongation facto
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(4)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
CBP-KK1, monoclonal
application:
IP
WB
WB
faj reaktivitás:
bacteria, Saccharomyces cerevisiae
technika/technikák:
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
citations:
96
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
CBP-KK1, monoclonal
faj reaktivitás
bacteria, Saccharomyces cerevisiae
faj reaktivitás (homológia által előrejelzett)
mammals
gyártó/kereskedő neve
Upstate®
technika/technikák
immunoprecipitation (IP): suitable
western blot: suitable
izotípus
IgG1κ
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... EEF1A1(1915)
Általános leírás
Human eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The alpha 1 isoform is expressed in brain, placenta, lung, liver, kidney, and pancreas. The other isoform (alpha 2) is expressed in brain, heart and skeletal muscle.
The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer.
The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer.
Egyediség
This antibody recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa. Occasionally, a 38 kDa protein is also detected in A431 cell lysate.
Immunogén
Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography. Clone CBP-KK1.
Alkalmazás
Detect EF1α using this Anti-EF1α Antibody, clone CBP-KK1 validated for use in IP & WB.
Immunoprecipitation:
4 µg of a previous lot immunoprecipitated EF1α from an A431 RIPA cell lysate.
4 µg of a previous lot immunoprecipitated EF1α from an A431 RIPA cell lysate.
Research Category
Protein Trafficking
Protein Trafficking
Research Sub Category
RNA Metabolism & Binding Proteins
RNA Binding Protein (RBP)
RNA Metabolism & Binding Proteins
RNA Binding Protein (RBP)
Minőség
Routinely evaluated on RIPA lysates from non-stimulated A431 cells, human Jurkat or mouse 3T3/A31.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected EF1α in RIPA lysates from non-stimulated A431 cells. Previous lots detected EF1α in human Jurkat and mouse 3T3/A31 RIPA cell lysates.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected EF1α in RIPA lysates from non-stimulated A431 cells. Previous lots detected EF1α in human Jurkat and mouse 3T3/A31 RIPA cell lysates.
Cél megnevezése
53 kDa
Fizikai forma
Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide.
Tárolás és stabilitás
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analízis megjegyzés
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Co-infection with the friend retrovirus and mouse scrapie does not alter prion disease pathogenesis in susceptible mice.
Leblanc, P; Hasenkrug, K; Ward, A; Myers, L; Messer, RJ; Alais, S; Timmes, A; Priola, SA; Priola, S
Testing null
CDK2 catalytic activity and loss of nuclear tethering of retinoblastoma protein in childhood acute lymphoblastic leukemia.
Schmitz, NM; Leibundgut, K; Hirt, A
Leukemia null
Molecular determinants for the tissue specificity of SERMs
Shang, Y. and Brown, M.
Science (New York, N.Y.), 295, 2465-2468 (2002)
Tatsuo Kido et al.
Genes, 1(2), 283-293 (2010-01-01)
TSPY is a Y-encoded gene that is expressed in normal testicular germ cells and various cancer types including germ cell tumor, melanoma, hepatocellular carcinoma, and prostate cancer. Currently, the correlation between TSPY expression and oncogenic development has not been established
S R Kimball et al.
The Journal of biological chemistry, 274(17), 11647-11652 (1999-04-17)
Regulation of translation of mRNAs coding for specific proteins plays an important role in controlling cell growth, differentiation, and transformation. Two proteins have been implicated in the regulation of specific mRNA translation: eukaryotic initiation factor eIF4E and ribosomal protein S6.
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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