Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Advanced healthcare materials (2015-06-20)

Timothy M O'Shea, Matthew J Webber, Alex A Aimetti, Robert Langer

ZUSAMMENFASSUNG

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

D2650

Sigma-Aldrich

Dimethylsulfoxid, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

D8418

Sigma-Aldrich

Dimethylsulfoxid, for molecular biology

D2438

Sigma-Aldrich

Dimethylsulfoxid, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

D4540

Sigma-Aldrich

Dimethylsulfoxid, ≥99.5% (GC), suitable for plant cell culture

276855

Sigma-Aldrich

Dimethylsulfoxid, anhydrous, ≥99.9%

T9531

Sigma-Aldrich

D-(+)-Trehalose Dihydrat, ≥99% (HPLC), from Saccharomyces cerevisiae

270997

Sigma-Aldrich

Dichlormethan, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

H9892

Sigma-Aldrich

Salzsäure -Lösung, 1.0 N, BioReagent, suitable for cell culture

21115

Sigma-Aldrich

Calciumchlorid -Lösung, BioUltra, for molecular biology, ~1 M in H₂O

H1758

Sigma-Aldrich

Salzsäure, 36.5-38.0%, BioReagent, for molecular biology

322415

Sigma-Aldrich

Methanol, anhydrous, 99.8%

T0167

Sigma-Aldrich

D-(+)-Trehalose Dihydrat, from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

C5670

Sigma-Aldrich

Calciumchlorid, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

S7388

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N-Succinyl-Ala-Ala-Pro-Phe p-Nitroanilid

T5251

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D-(+)-Trehalose Dihydrat, ≥98.5% (HPLC), from Saccharomyces cerevisiae

T9449

Sigma-Aldrich

D-(+)-Trehalose Dihydrat, ≥99% (HPLC), from starch

41639

Sigma-Aldrich

Dimethylsulfoxid, BioUltra, for molecular biology, ≥99.5% (GC)

860336

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidin, ≥99%

2104

Supelco

Salzsäure -Lösung, volumetric, 0.1 M HCl (0.1N), endotoxin free

270989

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Ethylacetat, anhydrous, 99.8%

544833

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Aluminiumoxid, nanopowder, <50 nm particle size (TEM)

L4777

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Lipase-Acrylharz aus Candida antarctica, ≥5,000 U/g, recombinant, expressed in Aspergillus niger

499609

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Calciumchlorid, anhydrous, powder, 99.99% trace metals basis

295426

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Chlorwasserstoff, ReagentPlus^®, ≥99%

C3142

Sigma-Aldrich

αα-Chymotrypsin, (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

D9170

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Dimethylsulfoxid, PCR Reagent

T2885

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3,3′,5,5′-Tetramethylbenzidin, ≥98% (TLC)

702129

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Aluminiumoxid, nanoparticles, <50 nm particle size (DLS), 20 wt. % in isopropanol

84429

Sigma-Aldrich

Salzsäure -Lösung, ~6 M in H₂O, for amino acid analysis

D1435

Sigma-Aldrich

Dimethylsulfoxid, meets EP testing specifications, meets USP testing specifications