G5663

Glutathione S-Transferase from E. coli

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(e): Glutathione S-alkenetransferase, Glutathione S-alkyltransferase, Glutathione S-aralkyltransferase, Glutathione S-aryltransferase, Glutathione S-epoxidetransferase, RX: Glutathione R-transferase
• MDL-Nummer: MFCD01634957
• UNSPSC-Code: 12352204
• NACRES: NA.53

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualität: for molecular biology
• Form: buffered aqueous solution
• Mol-Gew.: 26 kDa
• Konzentration: >0.1 mg/mL; >1 unit/mL
• UniProt-Hinterlegungsnummer: P09488
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Angaben zum Gen: human ... GSTM1(2944)

Beschreibung

Allgemeine Beschreibung

Glutathione S-transferase (GST) catalyzes the addition of the glutathione thiol group to a suitable electrophilic species. Enzymatic activities are based on the conjugation of reduced glutathione in the presence of a second substrate.

Spezifität

Glutathione S-transferase (GST) catalyzes the addition of the glutathione thiol group to a suitable electrophilic species. Enzymatic activities are based on the conjugation of reduced glutathione in the presence of a
second substrate.

In ELISA, 0.5 μg of recombinant glutathione S-transferase is the minimum detectable level of enzyme with an anti-glutatione S-transferase, alkaline phosphatase conjugate.

In immunoblot, 50 ng of recombinant GST is the minimum detectable level of enzyme with an anti-glutathione S-transferase, alkaline phosphatase conjugate.

Anwendung

Glutathione S-Transferase (GST) from E. coli has been used in the standard curve preparation for the quantification of GST-V5H6 produced by enzyme-linked immunosorbent assay (ELISA).

Suitable for use in ELISA and Western blot applications.

Biochem./physiol. Wirkung

Glutathione S-transferases (GST) catalyzes the conjugation of reduced glutathione with several substrates, which leads to detoxification. It also serves as transport proteins.

Warnhinweis

Due to the sodium azide content, consult the SDS for information regarding hazards and safe handling practices.

Einheitendefinition

One unit will conjugate 1.0 micromole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per minute at pH 6.5 at 25 deg C.

Physikalische Form

GST is supplied as a solution in phosphate buffered saline containing 0.02% sodium azide. Every lot of material supplied will have 5 mg total enzyme, at a concentration greater than 0.1 mg/ml, typically 1.0 mg/ml.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable