SAE0152
Lisozima
free of DNA contaminants, suitable for Microbiome research, lyophilized powder, protein ≥90%, ≥40,000 units/mg protein
Sinônimo(s):
Mucopeptídeo N-acetilmuramoil-hidrolase, Muramidase
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About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.77
Produtos recomendados
fonte biológica
chicken egg white
Nível de qualidade
forma
powder
atividade específica
≥40000 units/mg protein
peso molecular
single-chain 14.3 kDa
Características
DNA free
técnica(s)
cell based assay: suitable
adequação
suitable for cell lysis
aplicação(ões)
cell analysis
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
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Descrição geral
Purified Lysozyme DNA free SAE0152 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges.1 It hydrolyzes b(1à 4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.2,3,4 The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan.
The enzyme is active over a broad pH range (6.0-9.0).
Lysozyme activity: ≥40,000 units/mg protein.
For isolation of nucleic acids, lysozyme has been used in the lysis peptidoglycan layer of bacterial cell walls.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential. This purified lysozyme preparation (SAE0152) is purified from chicken egg white, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ≥ 90% with the remainder (∼10%) being buffer salts such as sodium acetate and sodium chloride, and undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges.1 It hydrolyzes b(1à 4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.2,3,4 The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan.
The enzyme is active over a broad pH range (6.0-9.0).
Lysozyme activity: ≥40,000 units/mg protein.
For isolation of nucleic acids, lysozyme has been used in the lysis peptidoglycan layer of bacterial cell walls.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential. This purified lysozyme preparation (SAE0152) is purified from chicken egg white, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ≥ 90% with the remainder (∼10%) being buffer salts such as sodium acetate and sodium chloride, and undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Aplicação
- Lysozyme is used for the extraction of genomic DNA from bacterial cells.
- Lysozyme is used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
- Lysonzyme is used to prepare spheroplasts.
A enzima quebra as paredes celulares das bactérias; usada para preparar esferoplastos.
Ações bioquímicas/fisiológicas
A lisoenzima hidrolisa as ligações β(1→4) entre o ácido N-acetilmurâmico e os resíduos de N-acetil-D-glicosamina no peptidoglicano e entre resíduos de N-acetil-D-glicosamina na chitodextrina. Células gram-positivas são bastante susceptíveis a essa hidrólise, pois suas paredes celulares têm uma alta proporção de peptidoglicano. Bactérias gram-negativas são menos susceptíveis devido à presença de uma membrana externa e uma menor proporção de peptidoglicano. Contudo, essas células podem ser hidrolisadas na presença de EDTA que quela íons metálicos na membrana bacteriana externa.
A enzima fica ativa em um amplo intervalo de pH (6,0 a 9,0). Em pH 6,2, atividade máxima é observada ao longo de um intervalo de forças iônicas (0,02 a 0,100 M) que em pH 9,2 (0,01 a 0,06 M).
A enzima fica ativa em um amplo intervalo de pH (6,0 a 9,0). Em pH 6,2, atividade máxima é observada ao longo de um intervalo de forças iônicas (0,02 a 0,100 M) que em pH 9,2 (0,01 a 0,06 M).
Características e benefícios
Purified Lysozyme free SAE0152 undergoes strict quality control testing to ensure it will be Free of DNA contaminants, suitable for Microbiome research.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential.
Definição da unidade
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Artigos
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
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