10837059001
Roche
Lysozyme
from hen egg white
Sinônimo(s):
Mucopeptide N-acetylmuramoyl-hydrolase, Muramidase, lysozyme
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About This Item
Produtos recomendados
fonte biológica
chicken egg white
Nível de qualidade
Ensaio
≥95%
forma
solid
atividade específica
>23000 U/mg (Shugar units)
peso molecular
14.4 kDa
embalagem
pkg of 10 g
fabricante/nome comercial
Roche
pH ideal
6.0-7.0
absorção
3.9 at 280 nm (10 mg enzyme/ml)
Descrição geral
Muramidase, Mucopeptide N-acetylmuramoyl-hydrolase
Aplicação
Lysozyme has been used for:
- Cell wall degradation
- Preparation of protoplasts
- Bacteriolysis
- Pharmacology (anti-inflammatory effect on mucous membrane; healing of tissue; protection against bleeding; normalization of mucus secretion; dissolution of pyrogens; potentiation of antibiotics)
- Food and drinks (flavor enhancer)
- Sample preparation prior to isolation of nucleic acids
Lysozyme has been used in the monitoring of transcription by real-time -PCR and sample preparation.
Ações bioquímicas/fisiológicas
Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.
Definição da unidade
One Shugar unit is that quantity of enzyme in 1 ml of a suspension of Micrococcus luteus of pH 7.0 whose initial absorbance at a wavelength of 450 nm is 0.750 for a pathlength of 10 mm which causes the absorbance to decrease at the rate of 0.001 per minute, all measurements being carried out at a temperature of +25 °C.
Nota de preparo
Working solution: Lysozyme hydrochloride is readily soluble in water and buffer solutions but insoluble in organic solvents.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.
Reconstituição
Prepare a stock solution at a concentration of 50 mg/ml in dist. water. Dispense into aliquots and store at -15 to -25 °C. Discard each aliquot after use.
Armazenamento e estabilidade
Store at 2 to 8 °C. (Store dry!)
Outras notas
For life science research only. Not for use in diagnostic procedures.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
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