233155

Cleland′s Reagent

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%.

• Sinônimo(s): Cleland’s Reagent, DTT, Dithiothreitol, Dithiothreitol, DTT
• Fórmula empírica (Notação de Hill): C₄H₁₀O₂S₂
• Número CAS: 3483-12-3
• Peso molecular: 154.25
• Número MDL: MFCD00004877
• Código UNSPSC: 12161504
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥97% (titration)
• Formulário: powder
• adequação da reação: reagent type: reductant
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: white to off-white
• solubilidade: water: 30 mg/mL; ethanol: soluble
• traços de cátion: heavy metals: ≤1 ppm; heavy metals: <1 ppm
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: SC[C@H](O)[C@@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• chave InChI: VHJLVAABSRFDPM-IMJSIDKUSA-N

Descrição

Descrição geral

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations.

DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes. Protects protein sulfhydryls and restores enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL Reagent, Cat. No. 233157) can be used. Oxidized dithiothreitol: ≤0.5%.

Oxidized dithiothreitol: ≤0.5%.

Aplicação

• Cyclic nucleotide phosphodiesterase in Dictyostelium discoideum: interconversion of two enzyme forms.: Discusses the role of Cleland′s Reagent in differentiating forms of cyclic nucleotide phosphodiesterase, showcasing its utility in enzymatic studies and its broader implications in biochemistry research (Chassy BM, 1972).

Embalagem

Packaged under inert gas

Advertência

Toxicity: Harmful (C)

Reconstituição

Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Outras notas

Gailis, L. 1994. Eur. J. Pharmacol. 292, 39.
Lauriault, V.V.M., and O′Brien, P.J. 1991. Mol. Pharmacol.40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716.
Cleland, W.W. 1964. Biochemistry3, 480.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS05,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H302,H315,H318
• Declarações de precaução: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Classificações de perigo: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable