Enhancing biocompatibility of some cation selective electrodes using heparin modified bacterial cellulose.

Enhancing biocompatibility of some cation selective electrodes using heparin modified bacterial cellulose.

Carbohydrate polymers (2015-10-03)

Ibrahim H A Badr, R Abdel-Sattar, Sherif M A S Keshk

ZUSAMMENFASSUNG

Bacterial cellulose (BC) and heparin-modified bacterial cellulose (HBC) were utilized to enhance the biocompatibility of highly thrombogenic PVC-based potassium and calcium membrane electrodes. Three types of membrane electrodes were prepared: (1) conventional PVC electrode (control), (2) PVC-based electrode sandwiched with bacterial cellulose membrane (BC-PVC), and (3) PVC-based electrode sandwiched with heparin-modified bacterial cellulose membrane (HBC-PVC). The potentiometric response characteristics of the modified potassium and calcium membrane electrodes (BC-PVC and HBC-PVC) were compared with those of the control PVC-based potassium and calcium selective electrode, respectively. Response characteristics of the modified membrane electrodes were comparable to the control PVC membrane electrode. The platelet adhesion investigations indicated that (BC) and (HBC) layers are less thrombogenic compared to PVC. Therefore, use of BC or HBC would enable the enhancement of the biocompatibility of PVC-based membrane electrodes for potassium and calcium while practically maintaining the overall electrochemical performance of the PVC sensing film.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

T1503

Sigma-Aldrich

Trizma^® Base, Primary Standard and Buffer, ≥99.9% (titration), crystalline

S9763

Sigma-Aldrich

Natriumphosphat, ACS reagent, ≥99.0%

360589

Sigma-Aldrich

Tetrahydrofuran, contains 200-400 ppm BHT as inhibitor, ACS reagent, ≥99.0%

G8270

Sigma-Aldrich

D-(+)-Glukose, ≥99.5% (GC)

T6066

Sigma-Aldrich

Trizma^® Base, BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

G7021

Sigma-Aldrich

D-(+)-Glukose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

71640

Sigma-Aldrich

Natriumphosphat, puriss. p.a., ACS reagent, anhydrous, ≥99.0% (T)

27109

Sigma-Aldrich

Zitronensäure, meets analytical specification of Ph. Eur., BP, USP, E330, anhydrous, 99.5-100.5% (based on anhydrous substance)

D9434

Sigma-Aldrich

Dextrose, 97.5-102.0% anhydrous basis, meets EP, BP, JP, USP testing specifications

04276

Sigma-Aldrich

Natriumphosphat, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E 339, anhydrous, 98-100.5% (calc. to the dried substance)

84822

Sigma-Aldrich

Sebacinsäure-bis-2-ethylhexylester, ≥97.0% (GC)

T6687

Sigma-Aldrich

Tromethamin, meets USP testing specifications

T1378

Sigma-Aldrich

Sigma 7-9^®, ≥99% (titration), crystalline

G7528

Sigma-Aldrich

D-(+)-Glukose, ≥99.5% (GC), BioXtra

93362

Sigma-Aldrich

Trizma^® Base, BioUltra, for molecular biology, ≥99.8% (T)

93352

Sigma-Aldrich

Trizma^® Base, ≥99.0% (T)

S5136

Sigma-Aldrich

Natriumphosphat, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%

S3264

Sigma-Aldrich

Natriumphosphat, for molecular biology, ≥98.5% (titration)

115533

Sigma-Aldrich

1,1′-Carbonyldiimidazol, reagent grade

49139

Sigma-Aldrich

D-(+)-Glukose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)

T6791

Sigma-Aldrich

Trizma^® Base, BioXtra, pH 10.5-12.0 (1 M in H₂O), ≥99.9% (titration)

21860

Sigma-Aldrich

1,1′-Carbonyldiimidazol, ≥97.0% (T)

94046

Sigma-Aldrich

Natriumphosphat, zweibasisch -Lösung, BioUltra, 0.5 M in H₂O

V0627

Sigma-Aldrich

Valinomycin, ≥98% (TLC), ≥90% (HPLC)

W230618

Sigma-Aldrich

Zitronensäure, ≥99.5%, FCC, FG

T4661

Sigma-Aldrich

Trizma^® Base, ≥99.9% (titration), crystalline

47249

Supelco

D-(+)-Glukose, analytical standard

C2404

Sigma-Aldrich

Zitronensäure, anhydrous, suitable for cell culture, suitable for plant cell culture

676764

Sigma-Aldrich

Tetrahydrofuran, ACS reagent, ≥99.0%, contains 200-400 ppm BHT as inhibitor

27487

Sigma-Aldrich

Zitronensäure, BioUltra, anhydrous, ≥99.5% (T)