Common Markers and Small Molecule Inhibitors in Golgi Studies.

Common Markers and Small Molecule Inhibitors in Golgi Studies.

Methods in molecular biology (Clifton, N.J.) (2022-12-14)

Sarah Bui, Drew Stark, Jie Li, Jianchao Zhang, Yanzhuang Wang

ZUSAMMENFASSUNG

In this chapter, we provide a detailed guide for the application of commonly used small molecules to study Golgi structure and function in vitro. Furthermore, we have curated a concise, validated list of endomembrane markers typically used in downstream assays to examine the consequent effect on the Golgi via microscopy and western blot after drug treatment. This chapter will be useful for researchers beginning their foray into the field of intracellular trafficking and Golgi biology.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

F0895

Sigma-Aldrich

FIBRONEKTIN AUS HUMANPLASMA, liquid, 0.1% (Solution), BioReagent, suitable for cell culture

T7765

Sigma-Aldrich

Tunicamycin aus Streptomyces sp.

C6628

Sigma-Aldrich

Chloroquin -diphosphat (Salz), powder or crystals, 98.5-101.0% (EP)

553210

Sigma-Aldrich

Rapamycin, ≥95% (HPLC), powder

A7250

Sigma-Aldrich

N-Acetyl-L-Cystein, Sigma Grade, ≥99% (TLC), powder

E1383

Sigma-Aldrich

Etoposid, synthetic, 95.0-105.0%, powder

P0046

Sigma-Aldrich

Pyocyanin, from Pseudomonas aeruginosa, ≥98% (HPLC)

569397

Sigma-Aldrich

Staurosporine, Streptomyces sp., Staurosporine, CAS 62996-74-, is a cell-permeable, potent, reversible, ATP-competitive inhibitor of protein kinases (IC₅₀ = 7, 20, 1.3, 0.7, & 8.5 nM for PKA, CAMK, MLCK, PKC, & PKG, respectively).

A8456

Sigma-Aldrich

4-(2-Aminoethyl)benzolsulfonylfluorid -hydrochlorid, ≥97.0% (HPLC)

474787

Sigma-Aldrich

MG-132, ≥95 % durch HPLC, Potent, reversible, and cell-permeable proteasome inhibitor (K_i = 4 nM).

344827

Sigma-Aldrich

Fucosyltransferase-Inhibitor, 2F-Peracetyl-Fucose, 2F-Peracetyl-Fucose is a cell-permeable fluorinated fucose derivative that acts as an inhibitor of fucosyltransferases following its uptake and metabolic transformation into a GDP-fucose mimetic.

SML0506

Sigma-Aldrich

LY-411575, ≥97% (HPLC)

654380

Sigma-Aldrich

Tunicamycin, Streptomyces lysosuperficus

196419

Millipore

BAPTA/AM, Membrane-permeable form of BAPTA.

L5288

Sigma-Aldrich

Latrunculin B aus Latruncula magnifica, ≥80% (HPLC), solid

L6785

Sigma-Aldrich

Lactacystin, ≥90% (HPLC)

11004638001

Roche

Chymostatin

203290

Sigma-Aldrich

Bisindolylmaleimid I, A highly selective, cell-permeable, and reversible protein kinase C (PKC) inhibitor (IC₅₀ = 10 nM) that is structurally similar to staurosporine.

I2764

Sigma-Aldrich

ML-7, powder

K1385

Sigma-Aldrich

KN-93, ≥98% (HPLC)

E3652

Sigma-Aldrich

Epoxomicin, ≥95% (HPLC), solid

11004646001

Roche

Antipain -dihydrochlorid aus microbieller Quelle, crystalline, suitable for cell culture, pkg of 10 mg

579053

Sigma-Aldrich

InSolution TAPI-1, Calbiochem, InSolution, ≥97%, inhibits MMPs and TACE

565749

Sigma-Aldrich

β-Secretase Inhibitor II, The β-Secretase Inhibitor II, also referenced under CAS 263563-09-3, controls the biological activity of β-Secretase. This small molecule/inhibitor is primarily used for Neuroscience applications.

565771

Sigma-Aldrich

γ-Sekretase-Inhibitor X, InSolution, ≥90%, 1 mM

M3694

Sigma-Aldrich

Anti-α1,2-Mannosidase IA antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution