wavelength 595 nm total test time 2 min working reagent stability 18-26°C for 2 weeks dynamic range 50 mg/dL 0.5 g/L
Parameter
18-26 °C temperature 50 μL sample volume
Lagertemp.
2-8°C
Allgemeine Beschreibung
Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.
Anwendung
Total protein kit, micro has been used to determine:
total protein in the bacterial homogenate by Bradford method[1]
total protein content by the Bradford microassay from the tissue extracts[2]
soluble protein content by the Bradford method from leaves[4]
Biochem./physiol. Wirkung
Total protein kit, micro can be used to determine the concentration of proteins in solution.
Eignung
Geeignet für die Proteinbestimmung für Proben von bis zu 0.5 mg/mL.
Prinzip
Brilliant Blue G (Coomassie-Blau) reagiert mit Protein in einem Säure-Alkohol-Medium zu einem blauen Farbstoff-Protein-Komplex. Die Farbe, gemessen bei 595 nm, ist proportional zur Proteinkonzentration.
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