Here is the recommended protocol for cell sample preparation:
1. Homogenize cells with the heme reagent which contains NaOH and detergent which will effectively lyse the cells.
2. Let incubate for 5 min for the color to develop, then centrifuge to pellet any debris.
3. Transfer 250 µL of clear supernatant to a 96 well plate and measure the absorbance at 400 nm.
Alle Fotos(1)
Wichtige Dokumente
MAK316
Heme Assay Kit
sufficient for 250 colorimetric tests
Synonym(e):
Heme Quantification Kit
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
Größe auswählen
Ansicht ändern
1 KIT
€ 409,00
About This Item
UNSPSC-Code:
12161503
NACRES:
NA.84
Empfohlene Produkte
Nachweisverfahren
colorimetric
Relevante Krankheit(en)
hematological disorder
Lagertemp.
2-8°C
Verwandte Kategorien
Allgemeine Beschreibung
Heme is a member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm, and is a key prosthetic group for various essential proteins such as hemoglobin, cytochromes, catalases and peroxidases. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Heme determination is widely practiced by researchers of various blood diseases.[1][2]
Leistungsmerkmale und Vorteile
Compatible with high-throughput handling systems.
Eignung
Suitable for the quantitative determination of heme and evaluation of drug effects on heme metabolism in a variety of biological samples such as blood, plasma, serum, urine, and heme carrying enzymes.
Prinzip
Heme assay is based on an aqueous alkaline solution method, in which the heme is converted into a uniform colored form, producing a colorimetric (400 nm) result, directly proportional to the heme concentration in the sample. The optimized formulation reduces interference and exhibits high sensitivity. One mg/dL heme equals 15.3M, 0.001%, or 10ppm. The kit has a linear detection range of 0.6–125M heme in a 96 well format.
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1
Lagerklassenschlüssel
8B - Non-combustible corrosive hazardous materials
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
Lot/Batch Number
Die passende Version wird nicht angezeigt?
Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Brell Jennifer et al.
Communications biology, 3(1), 621-621 (2020-10-29)
Iron is essential for living cells. Uptake of iron-loaded transferrin by the transferrin receptor 1 (CD71, TFR) is a major but not sufficient mechanism and an alternative iron-loaded ligand for CD71 has been assumed. Here, we demonstrate that CD71 utilizes
Role of heme and heme-proteins in trypanosomatid essential metabolic pathways.
Tripodi K E, et al.
Enzyme Research, 2011 (2011)
Yu-Yin Liu et al.
Reproduction (Cambridge, England), 158(3), 257-266 (2019-07-13)
Endometriosis (EMS) is a chronic inflammatory disease characterized by the presence of extrauterine endometrial tissues. It has been previously reported that the refluxed blood containing viable endometrial tissues and the defective elimination of peritoneal macrophages in the pelvic cavity may
Hemopexin therapy reverts heme-induced proinflammatory phenotypic switching of macrophages in a mouse model of sickle cell disease.
Vinchi F, et al.
Blood, 127(4), 473-486 (2016)
Dietmar Hammerschmid et al.
Computational and structural biotechnology journal, 19, 1874-1888 (2021-05-18)
Globin-coupled sensors (GCS) usually consist of three domains: a sensor/globin, a linker, and a transmitter domain. The globin domain (GD), activated by ligand binding and/or redox change, induces an intramolecular signal transduction resulting in a response of the transmitter domain.
-
How do you use this product on cell culture sample? How to homogenize the sample prior of heme measurement?
1 answer-
Helpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
-
-
How does this kit work with cell culture sample? How do you homogenize the cells prior of measurement?
1 answer-
This kit was validated for use with serum, plasma and whole blood. Cell lysates have not been analyzed with this kit and no specific guidance is provided for this type of sample preparation.
Helpful?
-
-
Could I use distilled water instead of ultrapure? Can I use this kit to measure how much plasma free hemoglobin I have in a plasma sample?
1 answer-
Only ultra pure water should be used because it is filtered through a 0.22 μm filter which removes any bacteria that could interfere with the chemical reaction.
Helpful?
-
-
Does MAK316 work for mouse sera?
1 answer-
MAK316 is expected to work for all species and should be compatible with mouse sera samples, as well as with other sample types such as blood, serum, plasma, and urine.
Helpful?
-
-
how is this kit used
1 answer-
The kit is used according to the product protocol. Here is a link to that document:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/386/102/mak316bul.pdf
Helpful?
-
-
お世話になります。臨床研究でヒトの血栓を回収してホルマリン固定しております。遊離ヘムを染めたいと考えておりますが 可能でしょうか？また、検体を送れば染めていただけるのでしょうか？
1 answer-
This product is specifically tested for the quantitative determination of heme in various biological samples. Solid tissues are homogenized and lysed by the heme reagent which contains NaOH and a detergent. This would also apply to the clot sample resulting in the lysis of any intact RBCs. Given the principle of the kit, measuring only the free heme would not be possible. Currently, there is no product available to meet your requirements. Unfortunately, lab services are not offered for this particular application.
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.
