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H9039

Hämin

from bovine, ≥90%

Synonym(e):

Chlorhämin, Chloroprotoporphyrin-IX-Eisen(III), Ferriprotoporphyrin-IX-chlorid, Hämin(chlorid)

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Ihnen/SKUVerfügbarkeitPreis
1 g
Warenkorb auf Verfügbarkeit prüfen
€ 64,30
10 g
Warenkorb auf Verfügbarkeit prüfen
€ 384,00
100 g
Warenkorb auf Verfügbarkeit prüfen
€ 783,00

Über diesen Artikel

Empirische Formel (Hill-System):
C34H32ClFeN4O4
CAS-Nummer:
Molekulargewicht:
651.94
UNSPSC Code:
12352202
NACRES:
NA.61
PubChem Substance ID:
EC Number:
240-140-1
Beilstein/REAXYS Number:
5229914
MDL number:
Form:
powder
Assay:
≥90%
Biological source:
bovine

€ 64,30


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biological source

bovine

Quality Segment

assay

≥90%

form

powder

technique(s)

toxicology assay: suitable

solubility

1.4 M NH4OH: soluble 25 mg/mL

storage temp.

2-8°C

SMILES string

Cc1c(CCC(O)=O)c2cc3c(CCC(O)=O)c(C)c4cc5nc(cc6c(C)c(C=C)c(cc1n2)n6[Fe](Cl)n34)c(C=C)c5C

InChI

1S/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;

InChI key

BTIJJDXEELBZFS-HXFTUNQESA-K

General description

Heme is a small molecule present either free or bound to hemoglobin in the bloodstream of mammals. This is an alternative source of iron within the host that contains a porphyrin ring containing a Fe2+ ion called hemin. [1]

Application

Hemin was used to study conjugation, characterization and toxicity of lipophosphoglycan-polyacrylic acid conjugate for vaccination against leishmaniasis.[2]

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Dieser Artikel
512807732633741
biological source

bovine

biological source

Porcine

biological source

-

biological source

-

assay

≥90%

assay

≥96.0% (HPLC)

assay

97%

assay

-

technique(s)

toxicology assay: suitable

technique(s)

tissue culture: suitable, transfection: suitable

technique(s)

-

technique(s)

-

form

powder

form

powder or crystals

form

solid

form

solid

solubility

1.4 M NH4OH: soluble 25 mg/mL

solubility

-

solubility

-

solubility

0.1-1.0 M NaOH: soluble, 1 N NH4OH: soluble

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

15-25°C


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Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–9 of 9 Questions  
  1. Is the Hemin product from bovine, 90% Halal and Kosher certified?

    1 answer
    1. This Hemin product is not Halal or Kosher certified. A Halal or Kosher certified Hemin product is not currently offered.

      Helpful?

  2. Recently got 1G of the product - H9039. Do I have to dissolve in a solution?

    1 answer
    1. This product is supplied in powder form and needs to be reconstituted. It is soluble in 1.4 M ammonium hydroxide at 25 mg/mL. Please review the product data page at: https://www.sigmaaldrich.com/product/sigma/h9039

      Helpful?

  3. What is the solvent medium, to dissolve hemin stock powder and what is stock concentration of hemin (H9039)

    1 answer
    1. This product is tested for solubility at 25 mg/mL in 1.4 M NH4OH. According to a published article, this material is also soluble at 50 mg/mL in 1.0 M NaOH. Please see the link below to review this publication:
      https://journals.asm.org/doi/10.1128/iai.01924-05

      Helpful?

  4. What is the recommended method of sterilization in a culture medium with Hemin addition? Autoclaving at 112°C or filter sterilization?

    1 answer
    1. Stock solutions of Hemin are typically added to medium and autoclaved at 121 °C for 15-20 minutes. Stock solutions may also be filter sterilized and added aseptically to sterile medium.

      Helpful?

  5. I couln´t understand when you say: "There is no shelf life date assigned to this product. The guarantee of quality is for one year from the time of shipment for bottles stored as recommended." Then for Hemin - from bovine we will not have a Exp.date?

    1 answer
    1. A number of products are not included in expiration testing programs, as there is no indication to conclude they are unstable. The only date shown on the certificate of analysis will be the Release Date. For these products there is no stated Retest, Expiration or Use-by-Date Date. We recommend handling per defined conditions as printed in our product literature and website product descriptions. Products should be routinely inspected by the customer to ensure they perform as expected. For those products, only our standard warranty of 1 year from the date of shipment of products is applicable. For further information on our warranty please refer to terms and conditions of sale.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. What is the shelf life for Hemin, Product H9039?

    1 answer
    1. There is no shelf life date assigned to this product. The guarantee of quality is for one year from the time of shipment for bottles stored as recommended.

      Helpful?

  8. What are some applications for Hemin, Product H9039?

    1 answer
    1. Hemin-agarose has reportedly been prepared for use in affinity chromatography of heme-binding proteins.  See Tsutsui, K., Affinity chromatography of heme-binding proteins: Synthesis of hemin-agarose. Meth. Enzymol. 123, 331, (1986). Sigma supplies a hemin agarose, product H6390. Hemin can also be used as a component in culture media for the cultivation of bacteria, such as Leishmania tarentolae.

      Helpful?

  9. How stable is Hemin, Product H9039, in solution?

    1 answer
    1. Sigma-Aldrich has not evaluated the solution stability of hemin solutions.  Also, we have not found any information on solution storage in the literature. We recommend that solutions be prepared fresh.

      Helpful?

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