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Ficoll® PM 70

Ficoll® PM 70

Type 70

Synonym(e):

Poly-(saccharose-co-epichlorhydrin)

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About This Item

CAS-Nummer:
72146-89-5
MDL-Nummer:
MFCD00131071
UNSPSC-Code:
12352201
NACRES:
NA.25
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Typ

Type 70

Qualitätsniveau

200

Form

powder

Mol-Gew.

~70,000

Farbe

white

Löslichkeit

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

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Verwandte Kategorien

Allgemeine Beschreibung

Ficoll® PM70, a high molecular weight branched polysaccharide polymer, is believed that they do not contain any ionized groups. This sucrose-polymer is formed by the copolymerization of sucrose with epichlorhydrin.

Anwendung

Ficoll® PM 70 has been used:
  • as a macromolecule in a comparative study to evaluate its effect in vitrification solutions
  • as a supplement in the vitrification solution for the vitrification procedure of oocytes
  • as a component of low serum medium for human lung fibroblast cell culture to promote a crowded environment

Biochem./physiol. Wirkung

Ficoll is commonly used as a macromolecular constituent of vitrification solutions. Due to their dense branching, Ficolls also serve as ideal, nearly spherical molecular crowding agents.
FicollTM PM 70 is used as a macromolecular crowding agent in studies of cell volume signaling and protein refolding.[1]It may be used in tissue engineering and macromolecular conformation research for the development, evaluation, and use of macromolecular crowding (MMC) systems and configurations. Ficoll PM-70 can also be used in vitrification research.

Sonstige Hinweise

Nicht-ionisches synthetisches Polymer aus Saccharose
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Rechtliche Hinweise

Ficoll is a registered trademark of Cytiva

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

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Analysenzertifikate (COA)

Lot/Batch Number
0000401688
0000356212
0000352976
0000334002
0000327569

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

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Emmanuel Dauty et al.
Journal of molecular recognition : JMR, 17(5), 441-447 (2004-09-14)
Aqueous environments in living cells are crowded, with up to >50 wt% small and macromolecule-size solutes. We investigated quantitatively one important consequence of molecular crowding--reduced diffusion of biologically important solutes. Fluorescence correlation spectroscopy (FCS) was used to measure the diffusion
Nobuhiko Tokuriki et al.
Protein science : a publication of the Protein Society, 13(1), 125-133 (2003-12-24)
Unfolded states of ribonuclease A were used to investigate the effects of macromolecular crowding on macromolecular compactness and protein folding. The extent of protein folding and compactness were measured by circular dichroism spectroscopy, fluorescence correlation spectroscopy, and NMR spectroscopy in
M B Burg
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 10(5-6), 251-256 (2000-12-23)
The non-ideal properties of solutions containing high concentrations of macromolecules can result in enormous increases in the activity of the individual macromolecules. There is considerable evidence that macromolecular crowding and confinement not only occur in cells, but that these are
C M Checura et al.
Theriogenology, 67(5), 919-930 (2006-12-19)
This study was designed to evaluate vitrification procedures for in vitro matured bovine oocytes for efficient blastocyst production after warming, IVF and culture. A second goal was to replace serum as the macromolecular component of the vitrification solution, without compromising
M Al-Habori
The international journal of biochemistry & cell biology, 33(9), 844-864 (2001-07-20)
Macromolecular crowding has been proposed as a mechanism by means of which a cell can sense relatively small changes in volume or, more accurately, the concentration of intracellular solutes. According to the macromolecular theory, the kinetics and equilibria of enzymes
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Protokolle

Ficoll® 400 für die Gradientenzentrifugation

Ficoll®-400 ist ein hochverzweigtes Polymer, das durch Copolymerisation von Saccharose und Epichlorhydrin gebildet wird. Ficoll® 400 ist vollständig nicht-ionisch. Aufgrund der großen Anzahl an Hydroxylgruppen ist Ficoll® 400 sehr hydrophil und extrem wasserlöslich.

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

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    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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