GE17-0300-10
Ficoll® PM 400
Cytiva 17-0300-10, pack of 100 g
Synonym(e):
Density gradient medium
About This Item
Empfohlene Produkte
Form
solid
spray-dried
Verpackung
pack of 100 g
Hersteller/Markenname
Cytiva 17-0300-10
Farbe
White
Löslichkeit
soluble
Allgemeine Beschreibung
Leistungsmerkmale und Vorteile
- For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
- Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
- Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
- Better osmotic properties than sucrose.
- Preserves functional and morphological integrity.
- Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
- Does not penetrate biological membranes.
- Serves as the raw material for preparation of Ficoll-Paque gradients.
- Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Lagerklassenschlüssel
13 - Non Combustible Solids
Analysenzertifikate (COA)
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Artikel
This page shows methods to remove Percoll from a sample after centrifugation.
Density Marker Beads facilitate Percoll gradient monitoring in biological separations.
The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.
The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.
Protokolle
The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Zur Herstellung eines Percoll-Gradienten muss die Osmolalität von Percoll mit Kochsalzlösung oder Zellkulturmedium eingestellt werden, damit Percoll mit physiologischen Salzlösungen isotonisch wird.
Verwandter Inhalt
Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.
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