One unit of DNA equals 50 µg. Molecular biology grade TE buffer is suitable for diluting DNA.
D4889
Desoxyribonukleinsäure Natriumsalz aus E. coli Stamm B
Genomic, unsheared
Synonym(e):
DNA
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0.25 UNIT
€ 216,00
1 UNIT
€ 220,00
5 UNITS
€ 867,00
€ 216,00
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Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
297-226-7
NACRES:
NA.52
MDL number:
Biological source:
Escherichia coli
Form:
lyophilized powder
Solubility:
water: ~1 g/L
Storage temp.:
2-8°C
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Unterstützung erhaltenbiological source
Escherichia coli
grade
Molecular Biology
form
lyophilized powder
mol wt
>45 kb
solubility
water: ~1 g/L
storage temp.
2-8°C
Quality Level
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Verwandte Kategorien
1 of 4
Dieser Artikel | D9768 | D1626 | L3755 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade - | grade - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form solid | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt >45 kb | mol wt 31.5 × 103 kDa, 48 kb | mol wt - | mol wt - |
General description
Escherichia coli strain B is most widely studied DNA after k-12. The genomes of E coli strain B and k-12 show differences in the IS sequence elements. Strain B have B-islands and express O-specific side-chain LPS.[1] The product is unsheared genomic DNA.
Application
The DNAs are particularly useful for genomic analysis, including PCR, library construction in bacteriophage λ, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
Biochem/physiol Actions
50% GC; Tm = 90.5 °C
Preparation Note
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A260 units per mg DNA. % GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Analysis Note
Analyzed by gel electrophoresis and visualization after ethidium bromide staining, with single predominant band of 45 kB or greater.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
One unit will yield an A260 of 1.0 in 1.0 ml of 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and 1 mM EDTA (1 cm light path).
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flash_point_f
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flash_point_c
Not applicable
ppe
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Genomic comparisons among Escherichia coli strains B, K-12, and O157: H7 using IS elements as molecular markers
Schneider D, et al.
BMC Microbiology, 2(1), 18-18 (2002)
Mohamed El-Agamy Farh et al.
Journal of microbiology (Seoul, Korea), 55(10), 767-774 (2017-09-29)
Gram-staining-negative, uniflagellated, rod-shaped, designated as DCY110
Johan Sukweenadhi et al.
Antonie van Leeuwenhoek, 106(5), 935-945 (2014-08-30)
Strain DCY84(T), a Gram-stain positive, rod-shaped, aerobic, spore-forming bacterium, motile by means of peritrichous flagella, was isolated from humus soil from Yongin forest in Gyeonggi province, South Korea. Strain DCY84(T) shared the highest sequence similarity with Paenibacillus barengoltzii KACC 15270(T)
Rudolph Spangler et al.
PloS one, 4(9), e7010-e7010 (2009-09-16)
PCR in principle can detect a single target molecule in a reaction mixture. Contaminating bacterial DNA in reagents creates a practical limit on the use of PCR to detect dilute bacterial DNA in environmental or public health samples. The most
M Agarwal et al.
BJU international, 88(9), 868-870 (2002-02-20)
To investigate the possible role of Gardnerella vaginalis in interstitial cystitis (IC), using molecular methods to avoid difficulties with the culture and recovery of viable organisms, and the problems associated with the recovery of low numbers of culturable organisms. Thirty-three
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D4889 Sigma-Aldrich Deoxyribonucleic acid sodium salt from Escherichia coli strain B I have 1 Unit of this DNA. Just want to make sure if it =50ug. Also, Can i use TE buffer to dilute the DNA? thank you for your help.
1 answer-
Helpful?
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what is the absolute amount of DNA in this product is it 50µg?
1 answer-
This product is supplied in a unit size. 1 unit of any DNA = 50 µg.
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