N1531

Denaturation Solution

for Northern and Southern blotting

• Synonym(e): Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer
• NACRES: NA.25
• UNSPSC Code: 12161700

Eigenschaften

• grade: Molecular Biology
• Quality Segment: 200
• form: solution
• storage temp.: room temp

Beschreibung

General description

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

Application

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

Other Notes

0.5 N NaOH, 1.5 M NaCl

The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Danger
• Gefahrencodes: H290,H314
• Sicherheitshinweise: P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
• Lagerklasse: 8B - Non-combustible corrosive hazardous materials
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable