Comparing the 91C and 90C2 kits, the primary incubation for 91C is 15 minutes, while the 90C2 requires 30 minutes. These times do not encompass other necessary steps like prewarming. There are other quick kits available, such as Sudan Black B and Myeloperoxidase, which are used for staining neutrophils. When using the Sudan Black B or Myeloperoxidase kits, monocytes do stain but with less intensity, allowing for differentiation between the two cell types based on stain intensity. The same is true for the Naphthol AS-D chloroacetate procedure, where staining in monocytes is typically weak to absent.
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Wichtige Dokumente
91C
Spezifische Leukozyten-Naphthol-AS-D-Chloracetat-Esterase (Kit)
Synonym(e):
Leder-Färbung
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
UNSPSC-Code:
12352106
NACRES:
NA.47
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Empfohlene Produkte
Beschreibung
Kit formulated with all liquid reagents
Qualitätsniveau
Haltbarkeit
Expiry date on the label.
IVD
for in vitro diagnostic use
dilution
(for histology)
Anwendung(en)
hematology
histology
Versandbedingung
wet ice
Lagertemp.
2-8°C
Verwandte Kategorien
Anwendung
Esterasen sind in der Natur allgegenwärtig und umfassen eine Reihe verschiedener Enzyme, die auf bestimmte Substrate wirken. Mit dem 91C-Kit wird Naphthol-AS-D-Chloracetat enzymatisch durch eine „spezifische Esterase“ hydrolysiert und eine Naphtholverbindung freigesetzt. Diese geht anschließend eine Kupplungsreaktion mit einer Diazoniumverbindung ein, wodurch sich stark gefärbte Ablagerungen in den Bereichen der enzymatischen Aktivität bilden. Dieses Enzym gilt üblicherweise als spezifisch für Zellen, die von Granulozyten abstammen. Es kann zum Nachweis von Neutrophilen in peripherem Blut, in Knochenmark oder in Paraffinschnitten eingesetzt werden.
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1 - Skin Sens. 1 - STOT RE 2 Oral - STOT SE 3
Zielorgane
Kidney, Respiratory system
Zusätzliche Gefahrenhinweise
Lagerklassenschlüssel
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Is there a kit available for staining Naphthol AS-D chloroacetate that can be completed in less than 30 minutes from start to finish?
1 answer-
Helpful?
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Is Napthol AS-D Chloroacetate (Specific Esterase) Kit used for identifying neutrophils in Frozen Section tissue in pathology laboratories during surgeries? Also, is there a real-time protocol available for using this stain during surgeries?
1 answer-
There are 2 kits for Napthol AS-D Chloroacetate kit for the detection of specific esterase. The kit is more commonly used with peripheral blood or bone marrow, but it is also possible to use the kit with either paraffin or frozen sections. Tissue will have to be selected for analysis, and frozen sections would then need to be cut. Once the frozen sections are obtained, the procedure is identical to the start procedure performed in a traditional histology laboratory. to perform the staining and have the slide ready for analysis is 30 minutes or possibly slightly longer. The staining procedure takes about 30 minutes or longer. Warmed reagents at 37°C are needed. Including a known positive control, like a paraffin section, is necessary. Deparaffinizing and staining the slide with the frozen section is good practice. A water bath speeds up the process. Using just a hot air oven may require 30 minutes or more leading to 60 minutes. This may be unacceptable for human testing.
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What is the difference between 90C2-1KT and 91C-1KT?
1 answer-
The 90C2 and the 91C kits produce the same staining results and can be chosen based on the customer's preference. The 90C2 kit uses capsules and a diazonium salt for staining, while the 91C kit utilizes a stable base solution and requires the use of a sodium nitrite solution. The main differences between the two kits lie in the formulation, ease of use for the customer, and the hours needed for manufacturing. There may also be some variation in the length of the kit expiration dating.
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How many slides can be stained using kits 90C2 or 91C?
1 answer-
Kit 90C2 is designed for 10 uses, with each use requiring approximately 50 mL of staining reagent. This volume of solution is typically sufficient to stain from 1 to 13 or 14 slides, meaning the kit can stain a minimum of 10 slides or a maximum of 130-140 slides, with the average use falling between these two extremes. Similarly, Kit 91C is also designed for 10 uses, with each use requiring approximately 50 mL of staining reagent. This volume of solution is typically sufficient to stain from 1 to 13 or 14 slides. However, this kit is prepared with all liquid reagents. If staining is done using coverslips or placing the tissue sections on the very end of the glass slide, it might actually be possible to get 20 uses out of the kit. For reduced volume usage, simply scale the volume for each reagent by an equal amount proportional to what is used in the standard protocol.
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