B3051
Brucella-Bouillon-Basis
suitable for microbiology, NutriSelect® Plus
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About This Item
UNSPSC-Code:
41171607
NACRES:
NA.85
Empfohlene Produkte
Sterilität
non-sterile
Qualitätsniveau
Form
powder
Haltbarkeit
limited shelf life, expiry date on the label
Hersteller/Markenname
NutriSelect® Plus
Methode(n)
microbiological culture: suitable
pH-Wert
7.0±0.2 (25 °C)
Anwendung(en)
clinical testing
environmental
food and beverages
sample preparation
microbiology
Eignung
selective for Brucella spp.
selective for Campylobacter spp.
Verwandte Kategorien
Anwendung
Empfohlen für die Anreicherung, Isolierung und Kultivierung von Brucella Spezies.
Komponenten
Zusammensetzung (g/L)
Casein-Hydrolysat, enzymatisch 10.00
Tiergewebe, peptisch verdaut 10.00
Hefeextrakt 2.00
Dextrose 1.00
Natriumchlorid 5.00
Natriumhydrogensulfit 0.10
Casein-Hydrolysat, enzymatisch 10.00
Tiergewebe, peptisch verdaut 10.00
Hefeextrakt 2.00
Dextrose 1.00
Natriumchlorid 5.00
Natriumhydrogensulfit 0.10
Angaben zur Herstellung
Suspend 14.05 g of Brucella Broth Base in 500 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile 5% v/v heat inactivated (H1138) horse serum. Add polymyxin B (2,500 IU, Cat. No. 81334), bacitracin (12,500 IU, Cat. No. 11702), nystatin (50,000 IU, Cat. No. 74721), cycloheximide (50mg, Cat. No. 01810), nalidixic acid (2.5mg, Cat. No. 70162) and vancomycin (10mg, Cat. No. 94747) to make the medium selective. Mix well before pouring into sterile petri plates or tubes.
For isolation of the Campylobacter species add the rehydrated contents of one vial of Campylobacter Growth Supplement (Cat. No. C2847) and 5-7% defibrinated sheep blood to 500 ml of medium base. 500 ml of medium base. The Campylobacter Growth Supplement can also be made by mixing 125 mg sodium pyruvate (Cat. No. 15990), 125 mg sodium metabisulfite (Cat. No. 71928 )and 125 mg ferrous sulfate (Cat. No. 44970) in 2 ml of distilled water and filtering the solution through a sterile 0.4 mm filter to sterilize.
For isolation of the Campylobacter species add the rehydrated contents of one vial of Campylobacter Growth Supplement (Cat. No. C2847) and 5-7% defibrinated sheep blood to 500 ml of medium base. 500 ml of medium base. The Campylobacter Growth Supplement can also be made by mixing 125 mg sodium pyruvate (Cat. No. 15990), 125 mg sodium metabisulfite (Cat. No. 71928 )and 125 mg ferrous sulfate (Cat. No. 44970) in 2 ml of distilled water and filtering the solution through a sterile 0.4 mm filter to sterilize.
Fußnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Rechtliche Hinweise
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
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Journal of bacteriology, 66(4), 502-504 (1953-10-01)
M Boyle et al.
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A preliminary report on a selective medium for the culture of Brucella, including fastidious types.
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Artikel
Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide.
Verwandter Inhalt
Step-by-step protocol for generating apical-out human gut organoids for microbiome, ADME/Tox, viral and gastrointestinal related disease research. See the complete organoid culture protocol.
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