Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Fortfahren mit
H1138
Pferdeserum
Donor Herd, USA origin, Heat inactivated, sterile-filtered, suitable for cell culture
Synonym(e):
HS, Pferdeseren, Pferdeserum, Seren, Serum
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 mL | Warenkorb auf Verfügbarkeit prüfen | € 63,20 |
500 mL | Warenkorb auf Verfügbarkeit prüfen | € 192,00 |
Über diesen Artikel
Biological source:
horse serum
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
€ 63,20
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenbiological source
horse serum
Quality Segment
sterility
sterile-filtered
composition
Hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL Endotoxin
shipped in
dry ice
storage temp.
−20°C
Application
Pferdeserum wurde wie folgt verwendet:
- als Medienzusatz im Differenzierungsmedium zum Kultivieren der Phäochromazytom(PC12)-Zellen
- in der Immunzytochemie und Immunhistochemie
- zum Untersuchen der therapeutischen Wirkung einer Serumbehandlung auf die Kardiomyozytenviabilität
Preparation Note
Aus kontrollierten Beständen entnommen.
Hergestellt durch Erwärmen bei 56 °C für 30 Minuten.
Hergestellt durch Erwärmen bei 56 °C für 30 Minuten.
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Dieser Artikel | |||
|---|---|---|---|
| description Donor Herd, USA origin, Heat inactivated, sterile-filtered, suitable for cell culture | description USA origin, Donor herd, suitable for cell culture, suitable for hybridoma | description Donor herd, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma | description USA origin, sterile-filtered, suitable for cell culture |
| biological source horse serum | biological source horse serum | biological source horse serum | biological source sheep |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, cell culture | hybridoma: suitable | technique(s) cell culture | mammalian: suitable |
| impurities ≤10 EU/mL Endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL Endotoxin | impurities ≤100 EU/mL endotoxin |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Lagerklasse
10 - Combustible liquids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves
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What is the Department of Transportation shipping information for this product?
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Why is serum Heat-Inactivated (HI)?
1 answer-
Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
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