ROAMYGLL

Amyloglucosidase

from Aspergillus niger

• Synonym(e): Aspergillus niger, disaccharidase-type a-glucosidase
• EC-Nummer: 3.2.1.3 (BRENDA, IUBMB)
• UNSPSC-Code: 12352204

Eigenschaften

• Biologische Quelle: Aspergillus niger
• Qualitätsniveau: 100
• Form: lyophilized
• Spezifische Aktivität: 6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate); ~6 units/mg protein (At 25 °C with glycogen as the substrate.)
• Mol-Gew.: 97 kDa
• Verpackung: pkg of 3,500 U (11202367001); pkg of 500 U (11202332001)
• Hersteller/Markenname: Roche
• Optimaler pH-Wert: 4.6-4.8
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase.[1] It is synthesized by several Aspergillus genus species.[2] This exo-enzyme is one of the major industrial enzymes.[3] The stability of amyloglucosidase can be increased by immobilization.[3]

Spezifität

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.

Anwendung

Amyloglucosidase from Aspergillus niger is used for the determination of starch.[4]

Biochem./physiol. Wirkung

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose.[5][6] The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.[7]

Angaben zur Herstellung

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:

• Western blot: 1 to 10 μg/ml

Working solution: Tris-buffered saline containing 0.1% Tween 20.

Storage conditions (working solution): 2 to 8 °C

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P304 + P340 - P342 + P311 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash