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重要文件

X100

Sigma-Aldrich

Triton X-100

Triton™ X-100

laboratory grade

同義詞:

4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇, t-辛基苯氧基聚乙氧基乙醇, 聚乙二醇 辛基苯醚

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About This Item

線性公式:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS號碼:
Beilstein:
2315025
MDL號碼:
分類程式碼代碼:
12161900
PubChem物質ID:
NACRES:
NA.21
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生物源

synthetic

品質等級

等級

laboratory grade

描述

non-ionic

形狀

liquid

mp

6  °C (43 °F)

轉變溫度

cloud point 65 °C
pour point ~7 °C

溶解度

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

SMILES 字串

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI 密鑰

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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一般說明

Triton X-100具有溶血作用[1],可用于DNA提取。[2]
Triton X-100是一种常用的非离子型的表面活性剂和乳化剂,经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的洗涤剂,并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。还可通透活细胞膜用于转染。[3][4]

應用

Triton-X已用于:
  • 细胞通透化处理用于免疫荧光染色[5]
  • western blot分析中裂解缓冲液中的一种成分[5]
  • 制备免疫金标记的细胞切片用于电子显微镜的Tris盐缓冲液中的一种成分[5]

生化/生理作用

广泛使用的非离子型表面活性剂,用于在温和的非变性条件下回收膜组分。

特點和優勢

  • 适于细胞生物学和生化研究
  • 非离子型非变性去污剂
  • 优异的润湿剂和乳化剂
  • 多用表面活性剂

法律資訊

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

483.8 °F - closed cup

閃點(°C)

251 °C - closed cup

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)


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分析證明 (COA)

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文章

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

條款

本操作流程使用过氧化物酶和邻联二茴香胺在500nm处进行胆固醇氧化酶活性的测定。胆固醇氧化酶是一种以氧为受体而作用于供体的CH-OH基团的酶。

Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

蛋白質抗原分子量測定、蛋白質互作、酵素活性和轉譯後修飾的技術。

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Questions

1–10 of 16 Questions  
  1. How long is Triton X-100 stable at -80 degrees in buffer?

    1 answer
    1. At -80°C, Triton X-100 is generally stable, but the stability can depend on the specific buffer conditions and the concentration of the detergent. In most cases, Triton X-100 remains effective at low temperatures, but it is advisable to avoid repeated freeze-thaw cycles, as these can lead to changes in the detergent's properties and effectiveness. Please note that the shelf life after opening is dependent upon various factors which might affect the stability and, therefore, it is not guaranteed and needs to be determined by the end user.

      Helpful?

  2. If a sterilization of Triton X-100 or its dilutions (maximum 10% of Triton X-100 in the total volume) is needed to remove DNAse and RNAse, what's the best approach?

    1 answer
    1. Autoclaving undiluted Triton X-100 is generally not recommended for sterilization. However, dilute solutions, up to 10%, may be autoclaved. At certain
      concentrations, solutions may become cloudy, but dispersible above the cloud point. The solution will clarify with stirring upon cooling. Note that DNAse and RNAse-free products are currently available. Please see the links below to review these product options:
      https://www.sigmaaldrich.com/product/sigma/t8787
      https://www.sigmaaldrich.com/product/mm/648466
      https://www.sigmaaldrich.com/product/sigma/93443

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  4. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  5. What is the correct CAS number for Triton X-100, 9002-93-1 or 9036-19-5?

    1 answer
    1. CAS 9036-19-5 is a generic CAS number and is correct for Triton® X-100. With this number, neither the substitution pattern of the aromatic ring (para, meta, ortho), nor the amount of the repeating units of the oxyethylene units is specified or described. Triton® X-100 with CAS 9002-93-1 is also correct but it defines only the para-substitution.

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  6. Will heating Triton to reduce its viscosity have any negative impact on the product? Does heating negatively affect the activity of Triton?

    1 answer
    1. Triton X-100 can be frozen, thawed, and used. However, caution is advised when heating it above 50°C, as discoloration may occur. This note indicates that excessive heating can lead to some form of change in Triton X-100. It would be reasonable to assume that the recommended working range is from ambient temperature to 50°C (or slightly lower). If your process requires heating the solution to temperatures higher than 50°C, it is recommended to add Triton X-100 only after the temperature has dropped below 50°C or lower. If you need to work at temperatures exceeding 50°C, it is advisable to conduct solution stability studies to assess potential damage or breakdown of Triton X-100.

      Helpful?

  7. Is a concentration of 8x10^-5 mol/L of Triton X-100 harmful to metal (steel/aluminum) and silicon equipment when submerged in water?

    1 answer
    1. The laboratory has not specifically tested the corrosive properties of Triton X-100 on metal. However, according to a publication titled "Triton X-100 and Sodium Lauryl Sulfate Surfactants as Environmentally Friendly Corrosion Inhibitors for Steel in 0.5 M H2SO4" in Advances in Analytical Chemistry, Triton X-100 appears to act as a corrosion inhibitor for steel.

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  8. What are the NFPA hazard numbers for this?

    1 answer
    1. With the implementation of the Globally Harmonized System (GHS) of hazard classification in our safety data sheets (SDS), we decided to no longer maintain the National Fire Protection Association (NFPA) information in section 15 of the SDS. Therefore, we no longer provide updates to the NFPA ratings. Kindly review the SDS available at this link: https://www.sigmaaldrich.com/US/en/sds/sial/x100?userType=anonymous

      Helpful?

  9. Hi, Bought the X100-100ml but cant find what the concentration is. Is this 100%? Kind regards,

    1 answer
    1. The product is a neat or pure liquid, the concentration is 100 %.

      Helpful?

  10. What's the molar concentration of the TritonX 100?? How can I make a 50mM concentration from this??

    1 answer
    1. This product is supplied as a neat or pure liquid with a density of 1.07 g/mL and an average molecular weight of 625 g/mol. Assuming 100%, the molarity is 1.712 M. To make 1 L of a 50 mM solution from this, use 29.2 mL of the 1.712 M starting concentration.

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1–10 of 16 Questions  

Reviews

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  1. Lillehammer, Norway
    • Reviews 2
    • Vote 1
    5 out of 5 stars.

    M.Sc.

    We use the Product for surface cleaning of artworks. Works as planned.

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  2. 1 Ratings-Only Review

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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