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N3129

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

Synonym(s):

p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP

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1 g

Available to ship TODAYfromMILWAUKEE

$71.30
5 g

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$149.00
25 g
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$545.00

About This Item

Linear Formula:
O2NC6H4OP(O)(O-)2 · 2C6H11NH3+
CAS Number:
Molecular Weight:
417.44
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
257-949-0
MDL number:
Beilstein/REAXYS Number:
6553276

$71.30


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Product Name

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate

Quality Segment

assay

≥97% anhydrous basis

form

powder

solubility

water: 10 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2

InChI key

KXNNUYCXODNFBD-UHFFFAOYSA-N

Application

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

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This Item
73737N3254N3002
assay

≥97% anhydrous basis

assay

≥97.0% (enzymatic)

assay

≥97% (enzymatic)

assay

≥99% (TLC)

solubility

water: 10 mg/mL, clear, colorless to faintly yellow

solubility

H2O: 0.1 g/mL, clear

solubility

water: 50 mg/mL, clear, colorless to light yellow

solubility

water: 20 mg/mL, clear to very slightly hazy

Quality Level

300

Quality Level

100

Quality Level

100

Quality Level

200

form

powder

form

powder or crystals

form

powder

form

powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C


Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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