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I4506

Sigma-Aldrich

IgG from human serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonim(y):

Human IgG

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Wybierz wielkość

10 MG
832,00 zł
50 MG
2960,00 zł
100 MG
4790,00 zł
500 MG
8350,00 zł

832,00 zł

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Wszystkie zdjęcia(3)

Wybierz wielkość

Zmień widok
10 MG
832,00 zł
50 MG
2960,00 zł
100 MG
4790,00 zł
500 MG
8350,00 zł

About This Item

Numer MDL:
MFCD00163923
Kod UNSPSC:
12352203
NACRES:
NA.46
metody:
ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable
application:
DIBA
ELISA
IEP
WB
citations:
228

832,00 zł

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Poproś o zamówienie zbiorcze

białko sprzężone

unconjugated

Poziom jakości

300

klasa czystości

reagent grade

Próba

≥95% (SDS-PAGE)

Formularz

essentially salt-free, lyophilized powder

skład

Protein, ≥90%

metody

ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable

temp. przechowywania

2-8°C

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Opis ogólny

Purified Human IgG is isolated from pooled normal human serum by fractionation and ion-exchange chromatography. The product is supplied as an essentially salt-free (less than 1% sodium), lyophilized powder.

Zastosowanie

IgG from human serum was used as a standard at a concentration of 1.274 mg/ml in ELISA to determine antibody responses to Porphyromonas gingivalis and Prevotella intermedia.[1] It was used for immunolabeling of Vero, BHK and PtK2 cells at a concentration of 0.2 mg/ml.[2] IgG from human serum was used as a standard in SDS-PAGE of anti-Shigella LPS antibodies.[3]

Działania biochem./fizjol.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.[4]

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

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Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
0000411605
0000410071
0000406823
0000406824
0000406825

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Youhei Egami et al.
Journal of cell science, 130(24), 4168-4179 (2017-11-09)
Phagosome formation is a complicated process that requires spatiotemporally regulated actin reorganization. We found that RhoC GTPase is a critical regulator of FcγR-mediated phagocytosis in macrophages. Our live-cell imaging revealed that RhoC, but not RhoA, is recruited to phagocytic cups
Nils A Brechmann et al.
Biotechnology progress, 35(3), e2775-e2775 (2019-01-11)
High capacity magnetic protein A agarose beads, LOABeads PrtA, were used in the development of a new process for affinity purification of monoclonal antibodies (mAbs) from non-clarified CHO cell broth using a pilot-scale magnetic separator. The LOABeads had a maximum
Katinka Döhner et al.
Molecular biology of the cell, 13(8), 2795-2809 (2002-08-16)
After fusion of the viral envelope with the plasma membrane, herpes simplex virus type 1 (HSV1) capsids are transported along microtubules (MTs) from the cell periphery to the nucleus. The motor ATPase cytoplasmic dynein and its multisubunit cofactor dynactin mediate
Egil S Blix et al.
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 21(5), 840-847 (2015-02-18)
Multiple myeloma (MM) is considered an incurable B cell malignancy, although many patients can benefit from high-dose therapy with autologous stem cell transplantation (ASCT) as a first-line treatment. In non-Hodgkin lymphoma (NHL), ASCT is usually performed after relapse with curative
Christopher Ashwood et al.
Journal of the American Society for Mass Spectrometry, 29(6), 1194-1209 (2018-04-01)
Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology. As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there
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Questions

1–10 of 13 Questions  

  1. Necesito saber la secuencia de la proteína o el número para buscarla en uniprot

    1 answer

    1. The protein sequence is not determined for this product. Human IgG has 4 subclasses: IgG1, IgG2, IgG3 and IgG4. These are present in serum at approximately 66%, 23%, 7% and 4%, respectively.

      Helpful?

  2. What is the isoelectric point of this

    1 answer

    1. This product is not tested on a lot-to-lot basis to determine the isoelectric point. However, historical data indicates an isoelectric point range of 6.1 - 8.5.

      Helpful?

  3. Is product I4506, because it is derived from human serum, considered to potential biohazard?

    1 answer

    1. As indicated in the corresponding Data Sheet at the link below: "BIOHAZARD: Handle as if capable of transmitting infectious agents". The source material is tested and found negative for antibody to HIV, antibody to HCV, and for HbsAG.

      Please see the link below to review the product data sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/203/i4506dat.pdf

      Helpful?

  4. What is the recommended storage method for item I4506, IgG from human serum, after reconstitution?

    1 answer

    1. After reconstitution, keep aliquots at 2-8°C for short-term use (up to 4 weeks) and store the remaining aliquots at -20°C.

      Helpful?

  5. What is the significance of absorbance at 280 nm (E280 1% = 14)?

    1 answer

    1. The absorbance at 280 nm (E280 1% = 14) indicates the percent extinction coefficient (ϵ1%).

      Helpful?

  6. what is the expected molecular weight under reducing conditions

    1 answer

    1. I4506, Human IgG, is a tetrameric protein consisting of 2 light chains and 2 heavy. When run on a reducing gel, The heavy chain will be detectable at ~50kDa, and the light chain at ~25kda.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. How should I store my solution of Product I4506, IgG from human serum?

    1 answer

    1. Solutions can be stored at -20 °C for at least 1 year.

      Helpful?

  9. What is the extinction coefficient forProduct I4506, human IgG?

    1 answer

    1. Protein concentration can be determined after reconstitution by absorbance at 280 nm (E280 (1%) = 14).  A 1% solution is 10 mg/mL.

      Helpful?

  10. Is Product I4506, IgG from human serum, tested for endotoxin?

    1 answer

    1. No. Product No. I4506 is not tested for endotoxin levels.

      Helpful?

1–10 of 13 Questions  

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