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G9136

Sigma-Aldrich

Porcine Gelatin

from porcine skin, Type A, powder, γ-irradiated, suitable for cell culture, BioXtra

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10 MG
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470,00 zł

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10 MG
470,00 zł

About This Item

Numer CAS:
9000-70-8
Numer WE:
232-554-6
Numer MDL:
MFCD00081638
Kod UNSPSC:
12352202
NACRES:
NA.75

470,00 zł

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Nazwa produktu

Gelatin from porcine skin, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

pochodzenie biologiczne

Porcine skin

Poziom jakości

200

sterylność

γ-irradiated

typ

Type A

linia produktu

BioXtra

Formularz

lyophilized powder

opakowanie

pkg of 10 mg

metody

cell culture | mammalian: suitable

pokrycie powierzchni

100‑200 μg/cm2

siła żelu

~300 g Bloom

rozpuszczalność

soluble 20 mg/mL, clear, colorless (in warm tissue culture medium)

Warunki transportu

ambient

temp. przechowywania

room temp

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Zastosowanie

This product is recommended for use as a cell culture substratum at 100-200 μg/cm2. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Komponenty

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Przestroga

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Uwaga dotycząca przygotowania

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g.

This product is a type A lyophilized powder, suitable for cell culture, with a gel strength of 300.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
SLCQ3744
SLCG7751
SLCC7170
SLCB7321
SLBZ6947

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Sigma-Aldrich

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Protokoły

Gelatin Coating Protocol for Cell Culture

This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.

Protokół powlekania żelatyną dla hodowli komórkowych

Ten protokół powlekania żelatyną do zastosowań w hodowli komórkowej zawiera informacje dotyczące rodzajów żelatyny, stężenia pokrycia powierzchni i wskazówki dotyczące optymalizacji.

Questions

  1. What is the molecular weight of this Gelatin?

    What is the molecular weight of this Gelatin?

    1 answer

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

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