G2500

Gelatin from porcine skin

Name: Gelatin from porcine skin
Brand: SIGMA

gel strength 300, Type A

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Numer CAS:

9000-70-8

MDL number:

MFCD00214058

EC Number:

232-554-6

NACRES:

NA.61

UNSPSC Code:

12352202

Form:

powder

Assay:

70-90% (biuret)

Biological source:

Porcine skin

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biological source

Porcine skin

Quality Level

200

type

Type A

assay

70-90% (biuret)

form

powder

technique(s)

cell culture | mammalian: suitable

gel strength

~300 g Bloom

solubility

H₂O: soluble 50 mg/mL

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Ta pozycja	G1890	39465	48724
Sigma-Aldrich G2500 Gelatin from porcine skin	Sigma-Aldrich G1890 Gelatin from porcine skin	Millipore 39465 Gelatin from porcine skin	Millipore 48724 Gelatin from porcine skin
assay 70-90% (biuret)	assay -	assay -	assay -
biological source Porcine skin	biological source Porcine skin	biological source Porcine	biological source Porcine
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable, electrophoresis: suitable	technique(s) -	technique(s) -
form powder	form powder	form powder	form powder
solubility H₂O: soluble 50 mg/mL	solubility H₂O: soluble 50 mg/mL, clear to hazy, faintly yellow	solubility H₂O: 67 mg/mL at 60 °C	solubility H₂O: 67 mg/mL at 50 °C, slightly hazy, faintly yellow, H₂O: 67 mg/mL at 60 °C
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100

General description

Gelatin is a hydrocolloid. Bovine or porcine bone and hide are its commercial sources.[1] It is a collagen derivative and comprises protein as a major component with minerals and water.[2]

Application

Gelatin from porcine skin has been used:

for coating glass-bottomed plates for culturing A375 cells and in gelatin degradation assay[1]
for coating glass slides in the histological studies with brain sections[3]
in the synthesis of gelatin methacrylate (GelMA) prepolymer for encapsulating cells on to 3D printed hydrogels[4]
to prepare thiol-functionalized gelatin (GelSH) hydrogel for 3D projection bioprinting[5]

Gelatin from porcine skin is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Biochem/physiol Actions

Gelatin polymer is an alternative biomaterial to collagen for cell and tissue culture.[2] It is a much sorted gelling agent useful as a thickener and food ingredient. Gelatin is also used as a foaming and binding agent and as well as in the pharmaceutical industry.[1] It is biodegradable and biocompatible with biomedical applications especially in biomaterials for cardiac and vascular tissue regeneration.[2]

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gell strength of 300 and is soluble in water at 50 mg/mL.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Disclaimer

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

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Klasa składowania

11 - Combustible Solids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number

0000541531

0000527716

0000527717

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Presynaptic NR2A-containing NMDA receptors implement a high-pass filter synaptic plasticity rule.

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Tissue-mimicking gelatin scaffolds by alginate sacrificial templates for adipose tissue engineering.

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When adipose tissue (AT) is impaired by trauma or disease, AT engineering could provide a shelf-ready structural and functional restoration as alternative to current clinical treatments, which mainly aim at aesthetic replacement. Yet, the lack of an efficient vascular network

Golgi-Cox Staining Step by Step.

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Golgi staining remains a key method to study neuronal morphology in vivo. Since most protocols delineating modifications of the original staining method lack details on critical steps, establishing this method in a laboratory can be time-consuming and frustrating. Here, we

In vitro characterization of chitosan-gelatin scaffolds for tissue engineering.

Yan Huang et al.

Biomaterials, 26(36), 7616-7627 (2005-07-12)

Recently, chitosan-gelatin scaffolds have gained much attention in various tissue engineering applications. However, the underlying cell-matrix interactions remain unclear in addition to the scaffold degradation and mechanical characteristics. In this study, we evaluated (i) the degradation kinetics of chitosan and

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Powiązane treści

Product Information Sheet - G2500

Instructions

Product Information Sheet

Questions

1–3 of 3 Questions

Anonymous

2 years ago

What is the molecular weight of this Gelatin?

What is the molecular weight of this Gelatin?

1 answer
1. Technical Support
  
  2 years ago
  
  The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
  Bloom Number Average Molecular Mass (Da)
  50−125 (Low Bloom) 20,000−25,000
  175−225 (Medium Bloom) 40,000−50,000
  225−325 (High Bloom) 50,000−100,000
  Please see the link below to review the general datasheet for Gelatin:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf
  
  Helpful?
2 years ago

what is the amino group content of the product Gelatine from porcine skin G2500?

1 answer
1. Technical Support
  
  2 years ago
  
  The amino group content of Gelatin is not determined in-house.
  
  Helpful?
3 years ago

What's the molecular weight of this gelatin

1 answer
1. Technical Support
  
  3 years ago
  
  The average molecular weight is between 50,000−100,000 Da.
  
  Helpful?

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