This product is a mixture of the CA-I and CA-I isozymes. The ratio has not been determined.
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C3934
Carbonic Anhydrase from bovine erythrocytes
lyophilized powder, ≥2,000 W-A units/mg protein
Synonim(y):
Carbonate Dehydratase, Carbonate Hydrolyase
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100 MG
1940,00 zł
500 MG
7580,00 zł
1940,00 zł
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100 MG
1940,00 zł
500 MG
7580,00 zł
About This Item
1940,00 zł
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Poproś o zamówienie zbiorcze
Polecane produkty
pochodzenie biologiczne
bovine erythrocytes
Formularz
lyophilized powder
aktywność właściwa
≥2,000 W-A units/mg protein
masa cząsteczkowa
30 kDa
Zastosowanie
diagnostic assay manufacturing
temp. przechowywania
2-8°C
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Zastosowanie
Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn2+, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation.[1] The enzyme from Sigma has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO2 measurement.[2] It has also been used for the thermodynamic analysis of conformational changes in BCA.[3]
Działania biochem./fizjol.
Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications for various pathologies such as glaucoma, epilepsy and Parkinson′s disease.[4]
Definicja jednostki
One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)
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Inhibitor
Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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Certyfikaty analizy (CoA)
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Klienci oglądali również te produkty
Use of carbonic anhydrase in electrochemical biosensors for dissolved CO2
Cammaroto C, et al.
Sensors and Actuators B, Chemical, 48(1-3), 439-447 (1998)
Fraser J Moss et al.
The Journal of physiology, 598(24), 5821-5856 (2020-09-25)
According to the HCO 3 - metabolon hypothesis, direct association of cytosolic carbonic anhydrases (CAs) with the electrogenic Na/HCO3 cotransporter NBCe1-A speeds transport by regenerating/consuming HCO 3 - . The present work addresses published discrepancies as to whether
Heather N Hollowell et al.
Journal of biochemistry and molecular biology, 40(2), 205-211 (2007-03-31)
The stability curve - a plot of the Gibbs free energy of unfolding versus temperature - is calculated for bovine erythrocyte carbonic anhydrase in 150 mM sodium phosphate (pH = 7.0) from a combination of reversible differential scanning calorimetry measurements
Angelina M Dichiera et al.
The Journal of experimental biology, 223(Pt 22) (2020-11-28)
Oxygen (O2) and carbon dioxide (CO2) transport are tightly coupled in many fishes as a result of the presence of Root effect hemoglobins (Hb), whereby reduced pH reduces O2 binding even at high O2 tensions. Red blood cell carbonic anhydrase
A carbonic anhydrase gene is induced in the nodule primordium and its cell-specific expression is controlled by the presence of Rhizobium during development.
Coba de la Pe??a, et al.
The Plant Journal, 11, 407-420 (2019)
Protokoły
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
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Dear who is concern, I would like to know which isoform of carbonic anhydrase this is.
1 answer-
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Product C3934, Carbonate Dehydratase?
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Because there are a number of closely related isoforms, the molecular weight typically used is approximately 30 kDa. This product is expected to be a mixture of isoforms.
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What is the purity of Product C3934, Carbonate Dehydratase?
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Since C3934 is a mixture of isoforms, no purity claim is made. The product is packaged and offered based on its enzymatic activity.
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Can stock solutions of Product C3934, Carbonate Dehydratase, be made and stored?
1 answer-
Carbonic anhydrase solutions can be prepared in water or buffer at concentrations up to 10 mg/mL. It is common for there to be some particulates or haziness in the solution. No data on solution stability has been collected by Sigma-Aldrich, so we recommend solutions be prepared fresh for use.
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