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E2039
Chondroitinase AC from Flavobacterium heparinum
recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis
Synonim(y):
Chondroitin AC lyase
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
10 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3030,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥200 units/mg protein
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
3030,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócrecombinant
expressed in E. coli
Quality Segment
conjugate
(Glucosaminoglycan)
assay
≥90% (SDS-PAGE)
form
lyophilized solid
specific activity
≥200 units/mg protein
shipped in
dry ice
storage temp.
−20°C
Application
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
Highly purified to remove interferring β-glucuronidase and protease activities for use in the hydrolysis of chondroitin sulfate pror to HPLC analysis.
Other Notes
1 unit is defined as the amount of enzyme that will liberate 1.0 μmole per minute of unsaturated disaccharides from chondroitin sulfate A at pH 6.7 at 37 °C as measured by the change in A232. The εμΜ for the reaction product Δ-Di-4S (chondroitin sulfates A and B) is 5.1 and 5.5 for Δ-Di-6S (chondroitin sulfate C).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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Ta pozycja | |||
|---|---|---|---|
| specific activity ≥200 units/mg protein | specific activity - | specific activity 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate) | specific activity ≥200 units/mg solid |
| assay ≥90% (SDS-PAGE) | assay - | assay - | assay - |
| form lyophilized solid | form lyophilized powder (with BSA as stabilizer) | form lyophilized powder | form lyophilized powder |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in - |
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Klasa składowania
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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