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C2905

Sigma-Aldrich

Chondroitinase ABC from Proteus vulgaris

lyophilized powder, 0.3-3 units/mg solid

Synonim(y):

Chondroitin ABC Lyase

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About This Item

Numer CAS:
9024-13-9
Numer EC enzymu:
4.2.2.4 (BRENDA, IUBMB)
Numer WE:
232-777-9
Numer MDL:
MFCD00130795
Kod UNSPSC:
12352204
NACRES:
NA.54

pochodzenie biologiczne

bacterial (Proteus vulgaris)

Poziom jakości

300

białko sprzężone

(Glucosaminoglycan)

Postać

lyophilized powder

aktywność właściwa

0.3-3 units/mg solid

masa cząsteczkowa

~120 kDa by gel filtration

skład

Protein, ~35% biuret

Zastosowanie

diagnostic assay manufacturing

temp. przechowywania

−20°C

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Zastosowanie

Chondroitinase ABC from Sigma has been used to digest O-linked chondroitin sulfate-like glycosaminoglycan (CSGAG). The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and expression of a recombinant versican gene in mammalian systems. Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes. The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan.

Działania biochem./fizjol.

Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl (or (1-3)-α-L-iduronosyl) linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate. However, it acts slowly on hyaluronate. The molecular weight is approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid. The optimal temperature is 37 °C. 1 mM Zn2+ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.

Opakowanie

Packages based on chondroitinase C activity.

Definicja jednostki

One unit will liberate 1.0 μmole of a mixture of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose and 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate from shark cartilage, per min at pH 8.0 at 37 °C.

Postać fizyczna

Lyophilized powder containing Tris buffer salts

Uwaga dotycząca przygotowania

Reconstitute in a 0.01% bovine serum albumin aqueous (BSA) solution. Subsequent dilutions can be made into a buffer containing 50 mM Tris, pH 8.0, with 60 mM sodium acetate and 0.02% BSA. Solutions should be freshly prepared.

Inne uwagi

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

Nicolett Kanyo et al.
Scientific reports, 10(1), 22422-22422 (2021-01-01)
The glycocalyx is thought to perform a potent, but not yet defined function in cellular adhesion and signaling. Since 95% of cancer cells have altered glycocalyx structure, this role can be especially important in cancer development and metastasis. The glycocalyx
Chin Lik Tan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(17), 6289-6295 (2011-04-29)
Chondroitin sulfate proteoglycans (CSPGs) are upregulated after CNS lesions, where they inhibit axon regeneration. In order for axon growth and regeneration to occur, surface integrin receptors must interact with surrounding extracellular matrix molecules. We have explored the hypothesis that CSPGs
Mafalda Resende et al.
International journal for parasitology, 39(11), 1195-1204 (2009-03-28)
Individuals living in areas with high Plasmodium falciparum transmission acquire immunity to malaria over time and adults have a markedly reduced risk of contracting severe disease. However, pregnant women constitute an important exception. Pregnancy-associated malaria is a major cause of
Gion Fessel et al.
Matrix biology : journal of the International Society for Matrix Biology, 28(8), 503-510 (2009-08-25)
The glycosaminoglycan (GAG) dermatan sulfate and chondroitin sulfate side-chains of small leucine-rich proteoglycans have been increasingly posited to act as molecular cross links between adjacent collagen fibrils and to directly contribute to tendon elasticity. GAGs have also been implicated in
Y Zhang et al.
The Journal of biological chemistry, 273(33), 21342-21351 (1998-08-08)
Versican is a member of the large aggregating chondroitin sulfate proteoglycan family. We have expressed in NIH3T3 fibroblasts a recombinant versican mini-gene comprising the G1 and G3 domains and 15% of the CS domain. We observed that expression of the
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