C2867
Cytochrome c from equine heart
BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture
Synonim(y):
CYC, CytC, Cytochrome c from horse heart
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About This Item
Polecane produkty
pochodzenie biologiczne
horse heart
linia produktu
BioUltra
Próba
≥99% (SDS-PAGE)
Postać
powder
masa cząsteczkowa
12,384
warunki przechowywania
(Tightly closed Dry)
metody
cell culture | mammalian: suitable
rozpuszczalność
water: 10 mg/mL, dark red-brown
numer dostępu UniProt
temp. przechowywania
−20°C
informacje o genach
horse ... CYCS(100053958)
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Opis ogólny
Cytochrome C (Cyt C) is a nuclear DNA-encoded protein. It is derived from its precursor apo-cytochrome c and moves across the outer membrane of the mitochondria.
Research area: Apoptosis
Research area: Apoptosis
Zastosowanie
Cytochrome c from equine heart has been used to prepare a stock solution for synthesizing and calibrating biotinylated cytochrome c. It has also been used as a component of the reaction buffer to stain electron transport chain (ETC) complex IV and determine its activity using blue-native gel electrophoresis.
The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell.
Działania biochem./fizjol.
Cytochrome C (Cyt C) acts as a potent signaling molecule of apoptosis by activating caspase 3, a death protease. It plays a role in heme-binding with the help of cytochrome c heme lyase which facilitates its release into the mitochondrial intermembrane space. Cyt C also exhibits lipid-binding by interacting with cardiolipin, a phospholipid that initiates protein unfolding and activates the peroxidase function in Cyt C.
Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen
Uwaga dotycząca przygotowania
A further purification of C2506
Prepared using TCA.
Inne uwagi
View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.
Zastosowanie
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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Chromatograms
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