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Merck

C2506

Sigma-Aldrich

Cytochrome c from equine heart

≥95% (SDS-PAGE)

Synonim(y):

Cytochrome c from horse heart

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About This Item

Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352202
NACRES:
NA.61

pochodzenie biologiczne

horse heart

Poziom jakości

Próba

≥95% (SDS-PAGE)

Formularz

powder

masa cząsteczkowa

12,384

metody

cell based assay: suitable

rozpuszczalność

H2O: soluble 10 mg/mL

przydatność

suitable for molecular biology

numer dostępu UniProt

Zastosowanie

cell analysis

temp. przechowywania

−20°C

informacje o genach

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Zastosowanie

Cytochrome c has been used:
  • in the reduction of ferricytochrome c by xanthine oxidase-generated superoxide radicals
  • as an analyte by the single droplet deposition method using MALDI mass spectra
  • as a terminal electron acceptor in the assay of standard cytochrome c reductase activity
  • in cytochrome c oxidase histochemistry
  • in the measurement of cytochrome C oxidase activity
  • as a component of phosphate buffer for the detection and evaluation of complex IV activity using blue-native gel electrophoresis (BN-PAGE)
  • for detection of extracellular superoxide anion (ECSA) in isolated kidney phagocytes
The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell.

Działania biochem./fizjol.

Cytochrome c, released from the outer mitochondrial compartment into the cytosol aids in activation of caspase-9 and initiation of the apoptotic protease cascade. This process is facilitated through its interaction with apoptotic protease-activating factor-1 (APAF-1) and deoxyadenosine triphosphate (dATP).(3) Various studies suggest that measurement of cytochrome C level in serum of tumor patients can be considered as a potential marker for cell death in vivo.
Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen

Uwaga dotycząca przygotowania

Prepared using TCA.
This product in solution should be dark red to reddish brown when dissolved in water at 10 mg/mL.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A novel role of the mitochondrial permeability transition pore in (-)-gossypol-induced mitochondrial dysfunction
Warnsmann V, et al.
Mechanisms of Ageing and Development, 170, 45-58 (2018)
Enzyme-catalyzed free radical reactions with nicotinamide adenine nucleotides. II. Lactate dehydrogenase-catalyzed oxidation of reduced nicotinamide adenine dinucleotide by superoxide radicals generated by xanthine oxidase.
P C Chan et al.
The Journal of biological chemistry, 249(4), 1317-1319 (1974-02-25)
B C Hill
The Journal of biological chemistry, 266(4), 2219-2226 (1991-02-05)
The reaction of the electrostatic cytochrome c-cytochrome oxidase complex with oxygen is measured by transient absorption spectroscopy. The oxygen reaction is initiated by photolytic removal of CO from cytochrome oxidase, using a flash-pumped dye laser. The subsequent reaction of the
Vittorio Pandini et al.
The Journal of biological chemistry, 277(50), 48463-48471 (2002-10-09)
Toxoplasma gondii possesses an apicoplast-localized, plant-type ferredoxin-NADP(+) reductase. We have cloned a [2Fe-2S] ferredoxin from the same parasite to investigate the interplay of the two redox proteins. A detailed characterization of the two purified recombinant proteins, particularly as to their
?-1,3 glucan derived from Euglena gracilis and Algamune? enhances innate immune responses of red drum (Sciaenops ocellatus L.)
Yamamoto FY, et al.
Fish & Shellfish Immunology, 77, 273-279 (2018)

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