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Merck

A8311

Sigma-Aldrich

Antifoam 204

aqueous emulsion for bacterial and mammalian systems

Synonim(y):

organic antifoam

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About This Item

Numer MDL:
Kod UNSPSC:
12161901
NACRES:
NA.85

Poziom jakości

Postać

emulsion (aqueous)

stężenie

100% (organic)

gęstość

1.01 g/mL at 25 °C

Zastosowanie

microbiology

temp. przechowywania

room temp

przydatność

(Mammalian (suspension))
bacteria (fermentation)

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Opis ogólny

Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Zastosowanie

Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.
Antifoam 204 has been used:
  • in the expression and purification of PUL (PLAP, Ufd3p, and Lub1p) domain
  • in fed-batch fermentation
  • to prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growth
  • in the batch fermentation with mineral medium

Komponenty

Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.

Inne uwagi

Contains 100% active components and is a mixture of non-silicone organic defoamers in a polyol dispersion. Can be sterilized repeatedly.

Uwaga dotycząca przygotowania

For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.
The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.
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produkt powiązany

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves


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