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G1N350

Sigma-Aldrich

GenElute Mammalian Genomic DNA Miniprep Kits

sufficient for 350 purifications

Synonim(y):

Mammalian Genomic DNA Miniprep, animal gDNA prep, gDNA miniprep, mammalian gDNA extraction, mammalian gDNA isolation, mammalian gDNA prep, mammalian gDNA purification, silica mammalian gDNA prep, Gen Elute

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1 KIT
4700,00 zł

4700,00 zł


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1 KIT
4700,00 zł

About This Item

Kod UNSPSC:
41105501
NACRES:
NA.55

4700,00 zł


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zastosowanie

sufficient for 350 purifications

Poziom jakości

metody

DNA purification: suitable

temp. przechowywania

15-25°C

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Opis ogólny

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient method to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.
The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Zastosowanie

GenElute Mammalian Genomic DNA Miniprep Kits has been used:
  • to extract DNA from fin samples[1]
  • to extract the BSTUI restriction enzyme from the DNA[2]
  • to isolate DNA from the blood samples extracted from caudal fin[3]
  • to extract tissue and hair follicle DNA[4]

The purified genomic DNA is ready for immediate use in downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning
  • ligation

Cechy i korzyści

  • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
  • Elution volume: 200 - 400 μl
  • Time required: 20 min after lysis
  • A260/A280 ratio: 1.6 - 1.9
  • Mechanical homogenization required: No

Zasada

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Inne uwagi

For additional information, please see www.sigma-aldrich.com/genomicdna.

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC
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Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki

  • C2112Column Preparation SolutionKarta charakterystyki

  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologyKarta charakterystyki

  • R6148RNase A solutionKarta charakterystyki

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

8A - Combustible corrosive hazardous materials

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Protokoły

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

Protokół zestawu do oczyszczania genomowego DNA ssaków GenElute opisuje izolację czystego DNA o wysokiej masie cząsteczkowej z różnych źródeł ssaków.

Poznaj podstawy blottingu Northern i Southern, wraz z protokołami i aplikacjami do przenoszenia makrocząsteczek na nośniki membranowe.

Zobacz wszystko

Powiązane treści

GenElute Mammalian Genomic DNA Purification Kit isolates pure DNA from mammalian cells using a silica-based membrane in spin columns.

Questions

  1. Is it possible to purchase the Elution Solution separately for the GenElute G1N350 kit, or can it be substituted with a different TE Buffer?

    1 answer
    1. Unfortunately, the elution buffer (B6803) is not available for individual purchase. However, we can provide the composition. It differs slightly from a standard TE buffer (typically 10 mM Tris and 1 mM EDTA pH 8.0) but can be easily prepared by combining Tris and EDTA with a slightly higher pH.

      Helpful?

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