The decline in activity over time due to storage conditions has not been determined. Other Proteinase K products may ship at ambient temperature, indicating little or no degradation of the material is expected. However, this items ships on ice. Maintaining the shipping and storage temperature at -20°C is recommended for this product in order to assure optimum performance.
Wszystkie zdjęcia(4)
Kluczowe dokumenty
P2308
Proteinase K from Tritirachium album
Proteinase K from Tritirachium album
lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
Synonim(y):
Endopeptidase K
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wybierz wielkość
5 MG
360,00 zł
10 MG
387,00 zł
25 MG
422,00 zł
100 MG
1650,00 zł
500 MG
5300,00 zł
1 G
7110,00 zł
P2308-5X1G
26 180,00 zł
360,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Poproś o zamówienie zbiorcze
Wybierz wielkość
Zmień widok
5 MG
360,00 zł
10 MG
387,00 zł
25 MG
422,00 zł
100 MG
1650,00 zł
500 MG
5300,00 zł
1 G
7110,00 zł
P2308-5X1G
26 180,00 zł
About This Item
Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54
360,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Poproś o zamówienie zbiorcze
Polecane produkty
klasa czystości
for molecular biology
Poziom jakości
linia produktu
BioUltra
Formularz
lyophilized powder
aktywność właściwa
≥30 units/mg protein
masa cząsteczkowa
28.93 kDa
skład
Protein, ≥90% biuret
zanieczyszczenia
≤1 ppm DNA
obecność zanieczyszczeń
DNAse, Nickase and RNAse, none detected
Warunki transportu
wet ice
temp. przechowywania
−20°C
Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów
Powiązane kategorie
Zastosowanie
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Działania biochem./fizjol.
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Definicja jednostki
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Ta strona może zawierać tekst przetłumaczony maszynowo.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
najczęściej kupowane z tym produktem
produkt podobny
produkt powiązany
zalecane rękawice chroniące przed rozpryskami
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organy docelowe
Respiratory system
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Wybierz jedną z najnowszych wersji:
Certyfikaty analizy (CoA)
Lot/Batch Number
Nie widzisz odpowiedniej wersji?
Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Mamoru Oshiki et al.
Frontiers in microbiology, 9, 830-830 (2018-05-15)
Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed
Margarita Zayas et al.
PLoS pathogens, 12(1), e1005376-e1005376 (2016-01-05)
Hepatitis C virus (HCV) nonstructural protein (NS)5A is a RNA-binding protein composed of a N-terminal membrane anchor, a structured domain I (DI) and two intrinsically disordered domains (DII and DIII) interacting with viral and cellular proteins. While DI and DII
Kumiko Muta et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 31(10), 1615-1623 (2016-05-18)
Although histone acetylation, an epigenetic modification, has been reported to be related to the progression of various diseases, its involvement in nephrosclerosis is unclear. Dahl salt-sensitive rats were used as a model of nephrosclerosis in this study. The rats were
Bahram Peivastegan et al.
BMC plant biology, 19(1), 262-262 (2019-06-19)
Stored potato (Solanum tuberosum L.) tubers are sensitive to wet conditions that can cause rotting in long-term storage. To study the effect of water on the tuber surface during storage, microarray analysis, RNA-Seq profiling, qRT-PCR and phytohormone measurements were performed
Sreenivas Adurthi et al.
Scientific reports, 7(1), 17289-17289 (2017-12-13)
Oestrogen controls Foxp3 expression in regulatory T cells (Treg cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in Treg cells remain unclear. We report that ERα ligand
Produkty
Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.
Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.
Proteinaza K pomaga w zastosowaniach biologii molekularnej poprzez trawienie białek strukturalnych, usuwanie nukleaz i izolowanie nienaruszonego genomowego DNA.
Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.
Protokoły
The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
Protokół zestawu do oczyszczania genomowego DNA ssaków GenElute opisuje izolację czystego DNA o wysokiej masie cząsteczkowej z różnych źródeł ssaków.
-
Hi, I'd like to know what happens if the lyophilized proteinase K (P238) is not stored at -20C for at least a week.
1 answer-
Helpful?
-
-
Is P2308 designed for antigen unmasking during IHC, and is there literature supporting this application?
1 answer-
Proteinase K has not been tested for use with IHC. Product P2308 is specifically tested for use in molecular biology, particularly for preparing cell lysates and releasing nucleic acids, as well as for PCR applications. While the enzyme has been used to digest hair (keratin) and may have other applications, there are no guarantees that P2308 would be suitable for antigen retrieval. No literature references stating that Proteinase K is commonly used for IHC were found.
Helpful?
-
-
Can P2308 be dissolved in water for nucleic acid preparation? If so, at what concentration? Thanks!
1 answer-
This product may be dissolved in water for nucleic acid preparations at 1 mg/mL.
Please see the link below to review the product datasheet which includes preparation instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/116/320/p2308dat.pdfHelpful?
-
-
How to prepare a solution of 20 mg/ml of pK (P2308-5MG)? In the section "Preparation Instructions" it mentions that I need to solve 1 mg in 1 ml of water. Does it means I'll have a final concentration of only 1 mg/ml?
1 answer-
The stock may also be prepared at 20 mg/ml concentration in 50 mM Tris-HCl, pH 8.0, containing 1 mM calcium chloride. This solution is stable for at least 12 months at 4°C. For additional information, please refer to the below publications where the product P2308 has been used.
1) https://www.nature.com/articles/s41598-017-17102-w#Sec9
2) https://www.frontiersin.org/articles/10.3389/fmicb.2018.00830/full#h3
3) https://elifesciences.org/articles/36187#s4Helpful?
-
-
How to prepare Proteinase K solution to use in antigen retrieval of formalin fixed paraffin embedded tissue sections for the purpose of paraffin immunoflorescence? Which is the diluent of choice to prepare the solution from powder form of proteinase K.
1 answer-
This product is not tested for use in antigen retrieval. The product 21627, IHC Select Proteinase K protocol may be applied to this product, P2308. To prepare a stock solution, dissolve Proteinase K, P2308, at 0.2mg/mL in 0.05M Tris solution, pH 7.5.
For antigen retrieval in FFPA tissue sections (deparaffinized) the recommended dilution of the stock solution is 1:30.
End users would need to optimize final dilutionHelpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the recommended pH range for Product P2308, Proteinase K?
1 answer-
Proteinase K is normally used in the pH range of 7.5-8.0.
Helpful?
-
-
How should I store solutions of Product P2308, Proteinase K?
1 answer-
Stock solutions in 40% glycerol of at least 20 mg/ml can be stored in 10 mM Tris-HCl buffer, pH 7.5.with 1 mM calcium acetate (see product P4850). Solutions are also stable at pH 8 at 4°C.
Helpful?
-
-
What amount of Product P2308, Proteinase K, should I use for nucleic acid preparations?
1 answer-
Amounts for typical usage is 50-200 μg/mL.
Helpful?
-
-
How should I prepare solutions of Product P2308, Proteinase K?
1 answer-
Solutions can be prepared in 25-50 mM Tris-HCl buffer, pH 8.0, containing 1-5 mM Calcium chloride.
Helpful?
-
Active Filters
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej
