Przejdź do zawartości
Merck

D9156

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from salmon testes

For hybridization

Synonim(y):

single-stranded template DNA

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych

Wybierz wielkość

1 ML
418,00 zł
5 ML
1240,00 zł
5 X 1 ML
1390,00 zł

418,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności


Wybierz wielkość

Zmień widok
1 ML
418,00 zł
5 ML
1240,00 zł
5 X 1 ML
1390,00 zł

About This Item

Numer CAS:
Numer MDL:
Kod UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52

418,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

pochodzenie biologiczne

fish testis (salmon)

Poziom jakości

klasa czystości

for molecular biology

Próba

9-11 mg/mL (DNA concentration)

Formularz

solution

stężenie

10 mg/mL in H2O

Warunki transportu

dry ice

temp. przechowywania

−20°C

Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów

Opis ogólny

A ready-to-use solution of high quality single-stranded template DNA isolated from the testes of salmon. The solution is supplied at a concentration of 9 - 12 mg/ml .

Zastosowanie

Deoxyribonucleic acid, single stranded from salmon testes is suitable for use as a blocking agent in Southern hybridizations. It was used for in situ hybridization histochemistry of bone marrow biopsy samples[1] and human blood monocyte subpopulations.[2]
For use as a blocking agent in Northern and Southern blotting.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.

Uwaga dotycząca przygotowania

This DNA is ethanol precipitated and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.

Inne uwagi

DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
Ta strona może zawierać tekst przetłumaczony maszynowo.

produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number

Nie widzisz odpowiedniej wersji?

Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

H W Ziegler-Heitbrock et al.
Blood, 79(2), 503-511 (1992-01-15)
Cytokine expression was analyzed in CD14++ regular monocytes and in the novel subset of CD14+/CD16+ small monocytes. Biologic activity for tumor necrosis factor (TNF), interleukin-1 (IL-1), and IL-6 in the supernatant of elutriator-enriched, cell sorter-purified small monocytes was about 10-fold
J W Said et al.
Blood, 90(11), 4278-4282 (1997-12-31)
We have recently demonstrated the presence of Kaposi's sarcoma-associated herpesvirus (KSHV) in cultured bone marrow (BM) stromal dendritic cells from all patients with myeloma studied. To show that these findings were not an artifact of tissue culture, we performed in
Marion Herbette et al.
PLoS genetics, 17(7), e1009662-e1009662 (2021-07-07)
Segregation Distorter (SD) is a male meiotic drive system in Drosophila melanogaster. Males heterozygous for a selfish SD chromosome rarely transmit the homologous SD+ chromosome. It is well established that distortion results from an interaction between Sd, the primary distorting
Maarten Holkers et al.
Nature methods, 11(10), 1051-1057 (2014-08-26)
Engineered sequence-specific nucleases and donor DNA templates can be customized to edit mammalian genomes via the homologous recombination (HR) pathway. Here we report that the nature of the donor DNA greatly affects the specificity and accuracy of the editing process
Zachary D Smith et al.
Nature, 511(7511), 611-615 (2014-08-01)
In mammals, cytosine methylation is predominantly restricted to CpG dinucleotides and stably distributed across the genome, with local, cell-type-specific regulation directed by DNA binding factors. This comparatively static landscape is in marked contrast with the events of fertilization, during which

Produkty

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Protokoły

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Poznaj podstawy blottingu Northern i Southern, wraz z protokołami i aplikacjami do przenoszenia makrocząsteczek na nośniki membranowe.

Questions

  1. What Salmon sp. D9156 is prepared from?

    1 answer
    1. The specific salmon species is not determined for this product. See the link below to review a sample Certificate of Origin for this material:
      https://www.sigmaaldrich.com/coo/undefined/D9156/SLBM7249V.

      Helpful?

Reviews

No rating value

Active Filters

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej